Recombinant人MEKK1 protein (ab104027)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • AccessionQ13233
    • 种属Human
    • 分子量155 kDa including tags
    • 氨基酸664 to 1512

技术指标

Our Abpromise guarantee covers the use of ab104027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性The Specific activity of ab104027 was determined to be 820 nmol/min/mg.
  • 应用

    SDS-PAGE

    Functional Studies

  • 纯度> 75 % SDS-PAGE.
    Purity was determined to be >75% by densitometry.
  • 形式Liquid
  • 补充说明

    ab126919 (Human MEK1 full length protein), ab126906 (Human ERK1 full length protein) and ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

     

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 0.01% Triton-X-100, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • M3K1_HUMAN
    • MAP3K1
    • MAPK/ERK kinase kinase 1
    • MAPKKK1
    • MEK kinase 1
    • Mekk
    • MEKK 1
    • MEKK1
    • Mitogen activated protein kinase kinase kinase 1
    • Mitogen activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase
    • Mitogen-activated protein kinase kinase kinase 1
    • SRXY6
    see all
  • 功能Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.
  • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
    Contains 1 RING-type zinc finger.
    Contains 1 SWIM-type zinc finger.
  • 翻译后修饰Autophosphorylated.
  • Information by UniProt

Recombinant human MEKK1 protein 图像

  • Kinase Assay demonstrating specific activity of ab104027.
  • SDS-PAGE showing ab104027 at approximately 155kDa.

Recombinant human MEKK1 protein (ab104027)参考文献

ab104027 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab104027.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"