Recombinant人MEK6 protein (ab95252)

概述

描述

  • 性质
    Recombinant
  • 来源
    Baculovirus
  • 氨基酸序列
    • 种属
      Human
    • 氨基酸
      2 to 334

技术指标

Our Abpromise guarantee covers the use of ab95252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, 100ng of inactive MAPK14 and 2 µM substrate (SER/THR 4) at room temperature for 1 hour. Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT.
  • 应用

    Functional Studies

    SDS-PAGE

  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Dual specificity mitogen activated protein kinase kinase 6
    • Dual specificity mitogen-activated protein kinase kinase 6
    • MAP kinase kinase 6
    • MAP2K6
    • MAPK/ERK kinase 6
    • MAPKK 6
    • MAPKK6
    • MEK 6
    • MEK6
    • Mitogen Activated Protein Kinase Kinase 6
    • MKK 6
    • MKK6
    • MP2K6_HUMAN
    • PRKMK6
    • Protein kinase mitogen activated kinase 6
    • protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6)
    • SAPK kinase 3
    • SAPKK-3
    • SAPKK3
    • SKK3
    • Stress-activated protein kinase kinase 3
    see all
  • 功能
    Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.
  • 组织特异性
    Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
  • 序列相似性
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Weakly autophosphorylated.
    Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt

图片

  • SDS-PAGE showing ab95252 at approximately 63.5kDa (5µg). Lane 2 shows molecular weight markers.
  • Image showing specific activity of ab95252.

文献

ab95252 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab95252.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册