The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, 100ng of inactive MAPK14 and 2 µM substrate (SER/THR 4) at room temperature for 1 hour. Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT.
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Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.