Recombinant人MCP2 protein (ab73834)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ser-Val.

技术指标

Our Abpromise guarantee covers the use of ab73834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性The biological activity was determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human THP-1 cells. Significant calcium mobilization is observed with >500ng/mL of recombinant human MCP2. Human MCP2 also induces dose dependent chemotaxis of human THP-1 cells with an ED50=30-100 ng/mL.
  • 应用

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.
    Purified by proprietary chromatographic techniques. Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
  • 形式Lyophilised
  • 补充说明For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Sterile solution containing no additives.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶Lyophilized from a concentrated (1mg/ml) sterile solution containing no additives. Reconstitute the lyophilized product in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

常规信息

  • 别名
    • Ccl8
    • CCL8_HUMAN
    • HC14
    • MCP-2
    • MCP-2(6-76)
    • Monocyte chemoattractant protein 2
    • Monocyte chemotactic protein 2
    • SCYA10
    • SCYA8
    • Small-inducible cytokine A8
    see all
  • 功能Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8.
  • 组织特异性Highest expression found in the small intestine and peripheral blood cells. Intermediate levels seen in the heart, placenta, lung, skeletal muscle, thymus, colon, ovary, spinal cord and pancreas. Low levels seen in the brain, liver, spleen and prostate.
  • 序列相似性Belongs to the intercrine beta (chemokine CC) family.
  • 翻译后修饰N-terminal processed form MCP-2(6-76) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • 细胞定位Secreted.
  • Information by UniProt

Recombinant human MCP2 protein (ab73834)参考文献

ab73834 has not yet been referenced specifically in any publications.

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