Recombinant人MARK4 protein (ab105211)



  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • AccessionQ96L34
    • 种属Human
    • 分子量104 kDa including tags
    • 氨基酸1 to 752


Our Abpromise guarantee covers the use of ab105211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性The Specific activity of ab105211 was determined to be 1050 nmol/min/mg.
  • 应用

    Western blot

    Functional Studies


  • 纯度> 90 % SDS-PAGE.
    Purity was determined to be >90% by densitometry.
  • 形式Liquid
  • 补充说明

    ab204854 (Cdc25C peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...


  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.


  • 别名
    • FLJ90097
    • KIAA1860
    • MAP / microtubule affinity regulating kinase 4
    • MAP / microtubule affinity regulating kinase 4L
    • MAP/microtubule affinity regulating kinase 4
    • MAP/microtubule affinity regulating kinase like 1
    • MAP/microtubule affinity-regulating kinase 4
    • MAP/microtubule affinity-regulating kinase-like 1
    • MARK 4
    • MARK 4L
    • MARK L1
    • MARK4
    • MARK4 serine / threonine protein kinase
    • MARK4 serine/threonine protein kinase
    • MARK4S
    • MARKL 1
    • MARKL1
    • Nbla00650
    • Putative protein product of Nbla00650
    see all
  • 功能Serine/threonine-protein kinase (PubMed:15009667, PubMed:14594945, PubMed:23666762, PubMed:23184942). Phosphorylates the microtubule-associated protein MAPT (PubMed:14594945, PubMed:23666762). Also phosphorylates the microtubule-associated proteins MAP2 and MAP4 (PubMed:14594945). Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles (PubMed:14594945, PubMed:25123532). Required for the initiation of axoneme extension during cilium assembly (PubMed:23400999). Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro (PubMed:23400999). Plays a role in cell cycle progression, specifically in the G1/S checkpoint (PubMed:25123532). Reduces neuronal cell survival (PubMed:15009667). Plays a role in energy homeostasis by regulating satiety and metabolic rate (By similarity). Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (By similarity). Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation (PubMed:23184942).
  • 组织特异性Ubiquitous. Isoform 2 is brain-specific (PubMed:11326310). Expressed at highest levels in brain and testis. Also expressed in heart, lung, liver, muscle, kidney and spleen (PubMed:14594945).
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 KA1 (kinase-associated) domain.
    Contains 1 protein kinase domain.
    Contains 1 UBA domain.
  • 发展阶段Expressed at all stages of the mitotic cell cycle.
  • 翻译后修饰Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.
    Phosphorylated at Thr-214 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39 (PubMed:14976552). Phosphorylated throughout the cell cycle (PubMed:25123532).
  • 细胞定位Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, cilium axoneme. Cytoplasm. Localized at the tips of neurite-like processes in differentiated neuroblast cells. Detected in the cytoplasm and neuropil of the hippocampus.
  • Information by UniProt

Recombinant human MARK4 protein 图像

  • SDS-PAGE showing ab105211 at approximately 104kDa.
  • The Specific activity of ab105211 was determined to be 1050 nmol/min/mg.

Recombinant human MARK4 protein (ab105211)参考文献

ab105211 has not yet been referenced specifically in any publications.

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