Recombinant人MAPRE1 protein (ab101824)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionQ15691
    • 种属Human
    • 序列MGSSHHHHHHSSGLVPRGSHMAVNVYSTSVTSDNLSRHDMLAWINESLQL NLTKIEQLCSGAAYCQFMDMLFPGSIALKKVKFQAKLEHEYIQNFKILQA GFKRMGVDKIIPVDKLVKGKFQDNFEFVQWFKKFFDANYDGKDYDPVAAR QGQETAVAPSLVAPALNKPKKPLTSSSAAPQRPISTQRTAAAPKAGPGVV RKNPGVGNGDDEAAELMQQVNVLKLTVEDLEKERDFYFGKLRNIELICQE NEGENDPVLQRIVDILYATDEGFVIPDEGGPQEEQEEY
    • 分子量32 kDa including tags
    • 氨基酸1 to 268
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab101824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Mass Spectrometry

  • 质谱法
    MALDI-TOF
  • 纯度> 90 % SDS-PAGE.
    ab101824 was purified using conventional chromatography techniques.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 2mM DTT, pH 8.0

常规信息

  • 别名
    • 5530600P05Rik
    • Adenomatous polyposis coli binding protein EB 1
    • Adenomatous polyposis coli binding protein EB1
    • AI462499
    • AI504412
    • APC binding protein EB 1
    • APC binding protein EB1
    • APC-binding protein EB1
    • AW260097
    • BIM1p
    • D2Ertd459e
    • EB 1
    • EB1
    • End binding protein 1
    • End-binding protein 1
    • fa01e12
    • fc23e11
    • fi33c06
    • MAPRE 1
    • MAPRE1
    • Mapre3
    • MARE1_HUMAN
    • MGC117374
    • MGC129946
    • MGC52508
    • Microtubule associated protein RP/EB family member 1
    • Microtubule-associated protein RP/EB family member 1
    • wu:fa01e12
    • wu:fc23e11
    • wu:fi33c06
    • zgc:55428
    • zgc:77807
    • zgc:85755
    see all
  • 功能May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
  • 组织特异性Ubiquitously expressed.
  • 序列相似性Belongs to the MAPRE family.
    Contains 1 CH (calponin-homology) domain.
    Contains 1 EB1 C-terminal domain.
  • 结构域Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
  • 细胞定位Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Associated with the microtubule network at the growing distal tip of microtubules. Also enriched at the centrosome.
  • Information by UniProt

Recombinant Human MAPRE1 protein 图像

  • 15% SDS-PAGE analysis of ab101824 (3µg)

Recombinant Human MAPRE1 protein (ab101824)参考文献

ab101824 has not yet been referenced specifically in any publications.

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