Recombinant人MAP3K9 protein (ab125554)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • AccessionP80192
    • 种属Human
    • 分子量77 kDa including tags
    • 氨基酸1 to 433

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技术指标

Our Abpromise guarantee covers the use of ab125554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性The specific activity of ab125554 was determined to be 116 nmol/min/mg.
  • 应用

    Western blot

    Functional Studies

    SDS-PAGE

  • 纯度> 85 % Densitometry.
    Purity was determined to be >85% by densitometry.
  • 形式Liquid
  • 补充说明

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • M3K9_HUMAN
    • Map3k9
    • MEKK9
    • Mitogen activated protein kinase kinase kinase 9
    • Mitogen-activated protein kinase kinase kinase 9
    • Mixed lineage kinase 1
    • Mixed lineage kinase 1 (tyr and ser/thr specificity)
    • MLK1
    • PRKE1
    see all
  • 功能Activates the JUN N-terminal pathway.
  • 组织特异性Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin.
  • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH3 domain.
  • 翻译后修饰Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site.
  • Information by UniProt

Recombinant human MAP3K9 protein 图像

  • SDS-PAGE analysis of ab125554.
  • The specific activity of ab125554 was determined to be 116 nmol/min/mg by Kinase Assay.

Recombinant human MAP3K9 protein (ab125554)参考文献

ab125554 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"