Recombinant人MAP3K8 protein (ab55700)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Human
    • 氨基酸30 to 397

技术指标

Our Abpromise guarantee covers the use of ab55700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific activity: 1012 nmol/min/mg
  • 应用

    SDS-PAGE

    Functional Studies

  • 形式Liquid
  • 补充说明

    ab43623 (Human ERK1 full length protein) and ab43614 (Human Myelin Basic Protein full length protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • c COT
    • Cancer Osaka thyroid oncogene
    • CCOT
    • COT
    • COT proto oncogene serine/threonine protein kinase
    • EST
    • ESTF
    • Ewing sarcoma transformant
    • FLJ10486
    • M3K8_HUMAN
    • MAP3K 8
    • MAP3K8
    • MEKK8
    • Mitogen activated protein kinase kinase kinase 8
    • Mitogen-activated protein kinase kinase kinase 8
    • Proto oncogene cCot
    • Proto-oncogene c-Cot
    • Serine/threonine protein kinase cot
    • Serine/threonine-protein kinase cot
    • TPL 2
    • TPL-2
    • TPL2
    • Tumor progression locus 2
    see all
  • 功能Required for TLR4 activation of the MEK/ERK pathway. Able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. Plays a role in the cell cycle. The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein.
  • 组织特异性Expressed in several normal tissues and human tumor-derived cell lines.
  • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • 发展阶段Isoform 1 is activated specifically during the S and G2/M phases of the cell cycle.
  • 翻译后修饰Autophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues.
  • 细胞定位Cytoplasm.
  • Information by UniProt

Recombinant human MAP3K8 protein 图像

  • Kinase activity plot using ab55700. Specific activity: 1012 nmol/min/mg.
  • SDS-PAGE analysis of ab55700, with molecular weight markers. Approximate molecular weight: 70kDa

Recombinant human MAP3K8 protein (ab55700)参考文献

ab55700 has not yet been referenced specifically in any publications.

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