Recombinant人MafA protein (ab112437)

概述

描述

  • 性质Recombinant
  • 来源Wheat germ
  • 氨基酸序列
    • AccessionQ8NHW3
    • 种属Human
    • 序列LEERFSDDQLVSMSVRELNRQLRGFSKEEVIRLKQKRRTLKNRGYAQSRF KRVQQRHILESEKCQLQSQVEQLKLEVGRLAKERDL
    • 分子量35 kDa including tags
    • 氨基酸222 to 308

相关产品

技术指标

Our Abpromise guarantee covers the use of ab112437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    ELISA

    Western blot

  • 形式Liquid
  • 补充说明Best use within three months from the date of receipt of this protein.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.79% Tris HCl, 0.31% Glutathione

常规信息

  • 别名
    • HMafA
    • Mafa
    • MAFA_HUMAN
    • Pancreatic beta cell specific transcriptional activator
    • Pancreatic beta-cell-specific transcriptional activator
    • RIPE3b1
    • Transcription factor MafA
    • Transcription factor RIPE3b1
    • v maf musculoaponeurotic fibrosarcoma oncogene homolog A
    • v maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian)
    • V-maf musculoaponeurotic fibrosarcoma oncogene homolog A
    see all
  • 功能Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context.
  • 序列相似性Belongs to the bZIP family. Maf subfamily.
    Contains 1 bZIP domain.
  • 翻译后修饰Ubiquitinated, leading to its degradation by the proteasome.
    Ser-14 and Ser-65 appear to be the major phosphorylation sites. Phosphorylated by MAPK13 on serine and threonine residues (Probable). Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation increases its transcriptional activity through the recruitment of the coactivator PCAF, is required for its transforming activity and leads to its degradation through an ubiquitin/proteasome-dependent pathway.
  • 细胞定位Nucleus. Detected in nuclei of pancreas islet beta cells.
  • Information by UniProt

Recombinant Human MafA protein 图像

  • 12.5% SDS-PAGE showing ab112437 at approximately 35.2kDa stained with Coomassie Blue.

Recombinant Human MafA protein (ab112437)参考文献

ab112437 has not yet been referenced specifically in any publications.

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