Recombinant人M-CSF protein (ab9694)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 分子量60 kDa

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技术指标

Our Abpromise guarantee covers the use of ab9694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    Functional Studies

    SDS-PAGE

  • 内毒素水平< 0.100 Eu/µg
  • 形式Lyophilised
  • 补充说明The ED50, determined by the dose-dependent stimulation of the proliferation of murine M-NSF-60 cells, is < 5.0 ng/ml, corresponding to a specific activity of > 2 x 105 units/mg
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 75mM Sodium chloride, 10mM Sodium phosphate, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶Reconstitute in water to a concentration of 0.1 - 0.5mg/ml.

常规信息

  • 别名
    • Colony stimulating factor 1
    • Colony stimulating factor 1 (macrophage)
    • Colony stimulating factor macrophage specific
    • CSF 1
    • CSF-1
    • CSF1
    • CSF1_HUMAN
    • Csfm
    • Lanimostim
    • M CSF
    • M-CSF
    • Macrophage colony stimulating factor
    • Macrophage Colony Stimulating Factor 1
    • MCSF
    • MGC31930
    • Processed macrophage colony-stimulating factor 1
    see all
  • 功能Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
  • 翻译后修饰Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
    Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.
  • 细胞定位Cell membrane and Secreted > extracellular space.
  • Information by UniProt

Recombinant human M-CSF protein 图像

  • Anti-M-CSF antibody (ab9693) at 0.2 µg/ml + Recombinant human M-CSF protein (ab9694) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

Recombinant human M-CSF protein (ab9694)参考文献

ab9694 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. This product is lyophilized from 3mM Tris, pH 8.5. M-CSF should be reconstituted in sterile ultra pure water (1mg/ml). Once the product in solution, then you can dilute into the media for storage. We have not perfo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"