重组人Lipoprotein lipase (ab115504)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 80% Densitometry
- Tags: DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, WB
描述
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产品名称
重组人Lipoprotein lipase
参阅全部 Lipoprotein lipase 蛋白酶 -
纯度
> 80 % Densitometry.
ab115504 is filtered (0.4 µm). -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
HVDYKDDDDKPAGADQRRDFIDIESKFALRTPEDTAEDTCHLIPGVAESV ATCHFNHSSKTFMVIHGWTVTGMYESWVPKLVAALYKREPDSNVIVVDWL SRAQEHYPVSAGYTKLVGQDVARFINWMEEEFNYPLDNVHLLGYSLGAHA AGIAGSLTNKKVNRITGLDPAGPNFEYAEAPSRLSPDDADFVDVLHTFTR GSPGRSIGIQKPVGHVDIYPNGGTFQPGCNIGEAIRVIAERGLGDVDQLV KCSHERSIHLFIDSLLNEENPSKAYRCSSKEAFEKGLCLSCRKNRCNNLG YEISKVRAKRSSKMYLKTRSQMPYKVFHYQVKIHFSGTESETHTNQAFEI SLYGTVAESENIPFTLPEVSTNKTYSFLIYTEVDIGELLMLKLKWKSDSY FSWSDWWSSPGFAIQKIRVKAGETQKKVIFCSREKVSHLQKGKAPAVFVK CHDKSLNKKSG -
预测分子量
52 kDa including tags -
氨基酸
28 to 475 -
标签
DDDDK tag N-Terminus
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描述
重组人Lipoprotein lipase
相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab115504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Western blot
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at -80°C.
pH: 7.50
Constituents: 0.24% Tris buffer, 0.29% Sodium chloride -
复溶Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.
常规信息
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别名
- EC 3.1.1
- EC 3.1.1.34
- HDLCQ11
see all -
功能
The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium. -
疾病相关
Defects in LPL are the cause of lipoprotein lipase deficiency (LPL deficiency) [MIM:238600]; also known as familial chylomicronemia or hyperlipoproteinemia type I. LPL deficiency chylomicronemia is a recessive disorder usually manifesting in childhood. On a normal diet, patients often present with abdominal pain, hepatosplenomegaly, lipemia retinalis, eruptive xanthomata, and massive hypertriglyceridemia, sometimes complicated with acute pancreatitis. -
序列相似性
Belongs to the AB hydrolase superfamily. Lipase family.
Contains 1 PLAT domain. -
翻译后修饰
Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity. -
细胞定位
Cell membrane. Secreted. Locates to the plasma membrane of microvilli of hepatocytes with triacyl-glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles. - Information by UniProt
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab115504 尚未被引用在任何文献中。