重组人LCAT蛋白(ab104359)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Tags: DDDDK tag N-Terminus
- Suitable for: SDS-PAGE
描述
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产品名称
重组人LCAT蛋白
参阅全部 LCAT 蛋白酶 -
纯度
> 95 % SDS-PAGE.
ab104359 is filtered (0.4 µm). Purity as determined by densitometric image analysis: >95% -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
HVDYKDDDDKPAGFWLLNVLFPPHTTPKAELSNHTRPVILVPGCLGNQLE AKLDKPDVVNWMCYRKTEDFFTIWLDLNMFLPLGVDCWIDNTRVVYNR SS GLVSNAPGVQIRVPGFGKTYSVEYLDSSKLAGYLHTLVQNLVNNGY VRDE TVRAAPYDWRLEPGQQEEYYRKLAGLVEEMHAAYGKPVFLIGHS LGCLHL LYFLLRQPQAWKDRFIDGFISLGAPWGGSIKPMLVLASGDNQ GIPIMSSI KLKEEQRITTTSPWMFPSRMAWPEDHVFISTPSFNYTGRD FQRFFADLHF EEGWYMWLQSRDLLAGLPAPGVEVYCLYGVGLPTPRTY IYDHGFPYTDPV GVLYEDGDDTVATRSTELCGLWQGRQPQPVHLLPLH GIQHLNMVFSNLTL EHINAILLGAYRQGPPASPTASPEPPPPE -
预测分子量
49 kDa including tags -
氨基酸
25 to 440 -
标签
DDDDK tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab104359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Lyophilized -
补充说明
Please filter the product by an appropriate sterile filter before using it in the cell culture.
Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C. -
Concentration information loading...
制备和贮存
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稳定性和存储
Store at -20°C. Avoid freeze / thaw cycle. Please see notes section.
pH: 7.50
Constituents: 0.242% Tris, 0.29% Sodium chloride -
复溶Add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile!
常规信息
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别名
- LCAT
- LCAT_HUMAN
- Lecithin cholesterol acyltransferase
see all -
功能
Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms. -
组织特异性
Expressed mainly in brain, liver and testes. Secreted into plasma and cerebral spinal fluid. In liver, expressed in HEPG2 hepatocytes. -
疾病相关
Defects in LCAT are the cause of lecithin-cholesterol acyltransferase deficiency (LCATD) [MIM:245900]; also called Norum disease. LCATD is a disorder of lipoprotein metabolism characterized by inadequate esterification of plasmatic cholesterol. Two clinical forms are recognized: familial LCAT deficiency and fish-eye disease. Familial LCAT deficiency is associated with a complete absence of alpha and beta LCAT activities and results in esterification anomalies involving both HDL (alpha-LCAT activity) and LDL (beta-LCAT activity). It causes a typical triad of diffuse corneal opacities, target cell hemolytic anemia, and proteinuria with renal failure.
Defects in LCAT are a cause of fish-eye disease (FED) [MIM:136120]; also known as dyslipoproteinemic corneal dystrophy or alpha-LCAT deficiency. FED is due to a partial LCAT deficiency that affects only alpha-LCAT activity. It is characterized by low plasma HDL and corneal opacities due to accumulation of cholesterol deposits in the cornea ('fish-eye'). -
序列相似性
Belongs to the AB hydrolase superfamily. Lipase family. -
翻译后修饰
O- and N-glycosylated. O-glycosylation on Thr-431 and Ser-433 consists of sialylated galactose beta 1-->3N-acetylgalactosamine structures. N-glycosylated sites contain sialylated triantennary and/or biantennary complex structures. -
细胞定位
Secreted. Secreted into blood plasma. Produced in astrocytes and secreted into cerebral spinal fluid. - Information by UniProt
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab104359 尚未被引用在任何文献中。