重组人LATS1/WARTS蛋白(ab125612)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: = 70% Affinity purified
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
描述
-
产品名称
重组人LATS1/WARTS蛋白 -
生物活性
The specific activity of ab125612 was determined to be 1.4 nmol/mg/min.
-
纯度
= 70 % Affinity purified.
Purified by affinity chromatography -
表达系统
Baculovirus infected Sf9 cells -
Accession
-
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
-
种属
Human -
序列
KEDESEKSYE NVDSGDKEKK QITTSPITVR KNKKDEERRE SRIQSYSPQA FKFFMEQHVE NVLKSHQQRL HRKKQLENEM MRVGLSQDAQ DQMRKMLCQK ESNYIRLKRA KMDKSMFVKI KTLGIGAFGE VCLARKVDTK ALYATKTLRK KDVLLRNQVA HVKAERDILA EADNEWVVRL YYSFQDKDNL YFVMDYIPGG DMMSLLIRMG IFPESLARFY IAELTCAVES VHKMGFIHRD IKPDNILIDR DGHIKLTDFG LCTGFRWTHD SKYYQSGDHP RQDSMDFSNE WGDPSSCRCG DRLKPLERRA ARQHQRCLAH SLVGTPNYIA PEVLLRTGYT QLCDWWSVGV ILFEMLVGQP PFLAQTPLET QMKVINWQTS LHIPPQAKLS PEASDLIIKL CRGPEDRLGK NGADEIKAHP FFKTIDFSSD LRQQSASYIP KITHPTDTSN FDPVDPDKLW SDDNEEENVN DTLNGWYKNG KHPEHAFYEF TFRRFFDDNG YPYNYPKPIE YEYINSQGSE QQSDEDDQNT GSEIKNRDLV YV -
预测分子量
95 kDa including tags -
氨基酸
589 to 1130
-
相关产品
-
Related Products
技术指标
Our Abpromise guarantee covers the use of ab125612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
Western blot
Functional Studies
SDS-PAGE
-
形式
Liquid -
补充说明
ab204882 (SGK peptide) can be utilized as a substrate for assessing kinase activity
This product was previously labelled as LATS1
-
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
-
别名
- h-warts
- Large tumor suppressor homolog 1
- LATS large tumor suppressor homolog 1
see all -
功能
Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function. -
组织特异性
Expressed in all adult tissues examined except for lung and kidney. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain. -
翻译后修饰
Autophosphorylated and phosphorylated during M-phase of the cell cycle. Phosphorylated by STK3/MST2 at Ser-909 and Thr-1079, which results in its activation. Phosphorylation at Ser-464 by NUAK1 and NUAK2 leads to decreased protein level and is required to regulate cellular senescence and cellular ploidy. -
细胞定位
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis. - Information by UniProt
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab125612 被引用在 1 文献中.
- Gan W et al. LATS suppresses mTORC1 activity to directly coordinate Hippo and mTORC1 pathways in growth control. Nat Cell Biol 22:246-256 (2020). PubMed: 32015438