Recombinant人KPNA5 protein (ab116831)

概述

描述

  • 性质Recombinant
  • 来源Wheat germ
  • 氨基酸序列
    • AccessionO15131
    • 种属Human
    • 序列MDAMASPGKDNYRMKSYKNKALNPQEMRRRREEEGIQLRKQKREEQLFKR RNVYLPRNDESMLESPIQDPDISSTVPIPEEEVVTTDMVQMIFSNNADQQ LTATQKFRKLLSKEPNPPIDQVIQKPGVVQRFVKFLERNENCTLQFEAAW ALTNIASGTFLHTKVVIETGAVPIFIKLLNSEHEDVQEQAVWALGNIAGD NAECRDFVLNCEILPPLLELLTNSNRLTTTRNAVWALSNLCRGKNPPPNF SKVSPCLNVLSRLLFSSDPDVLADVCWALSYLSDGPNDKIQAVIDSGVCR RLVELLMHNDYKVVSPALRAVGNIVTGDDIQTQVILNCSALPCLLHLLSS PKESIRKEACWTVSNITAGNRAQIQAVIDANIFPVLIEILQKAEFRTRKE AAWAITNATSGGTPEQIRYLVALGCIKPLCDLLTVMDSKIVQVALNGLEN ILRLGEQESKQNGIGINPYCALIEEAYGLDKIEFLQSHENQEIYQKAFDL IEHYFGVEEDDPSIVPQVDENQQQFIFQQQEAPMDGFQL
    • 分子量85 kDa including tags
    • 氨基酸1 to 539

技术指标

Our Abpromise guarantee covers the use of ab116831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    ELISA

    SDS-PAGE

    Western blot

  • 形式Liquid
  • 补充说明Protein concentration is above or equal to 0.05 mg/ml.
    Best use within three months from the date of receipt of this protein.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

常规信息

  • 别名
    • IMA5_HUMAN
    • Importin alpha 6
    • Importin subunit alpha 6
    • Importin subunit alpha-6
    • IPOA 6
    • IPOA6
    • Karyopherin alpha 5
    • Karyopherin alpha 5 importin alpha 6
    • Karyopherin subunit alpha 5
    • Karyopherin subunit alpha-5
    • KPNA 5
    • KPNA5
    • SRP 6
    • SRP6
    see all
  • 功能Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates nuclear import of STAT1 homodimers and STAT1/STAT2 heterodimers by recognizing non-classical NLSs of STAT1 and STAT2 through ARM repeats 8-9. Recognizes influenza A virus nucleoprotein through ARM repeat 7-9 In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
  • 组织特异性Testis.
  • 序列相似性Belongs to the importin alpha family.
    Contains 10 ARM repeats.
    Contains 1 IBB domain.
  • 结构域Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
    The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
    The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
  • 细胞定位Cytoplasm.
  • Information by UniProt

Recombinant Human KPNA5 protein 图像

  • 12.5% SDS-PAGE stained with Coomassie Blue showing ab116831

Recombinant Human KPNA5 protein (ab116831)参考文献

ab116831 has not yet been referenced specifically in any publications.

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