Recombinant人KPNA3 protein (ab114747)



  • 性质Recombinant
  • 来源Wheat germ
  • 氨基酸序列
    • AccessionO00505
    • 种属Human
    • 分子量37 kDa including tags
    • 氨基酸422 to 521


Our Abpromise guarantee covers the use of ab114747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用



    Western blot

  • 形式Liquid
  • 补充说明Protein concentration is above or equal to 0.05 mg/ml.
    Best used within three months from the date of receipt.
  • Concentration information loading...


  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl


  • 别名
    • SRP1 gamma
    • hSRP1
    • IMA4_HUMAN
    • Importin alpha 3
    • Importin alpha 3 subunit
    • Importin alpha 4
    • Importin alpha Q2
    • Importin subunit alpha-4
    • IPOA4
    • Karyopherin alpha 3
    • Karyopherin alpha 3 importin alpha 4
    • Karyopherin alpha 3 subunit
    • Karyopherin subunit alpha 3
    • Karyopherin subunit alpha-3
    • KPNA 3
    • Kpna3
    • Qip2
    • SRP1
    • SRP1-gamma
    • SRP1gamma
    • SRP4
    see all
  • 功能Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Recognizes NLSs of influenza A virus nucleoprotein probably through ARM repeats 7-9.
  • 组织特异性Ubiquitous. Highest levels in heart and skeletal muscle.
  • 序列相似性Belongs to the importin alpha family.
    Contains 10 ARM repeats.
    Contains 1 IBB domain.
  • 结构域Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
    The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
    The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt

Recombinant Human KPNA3 protein 图像

  • 12.5% SDS-PAGE showing ab114747 at approximately 36.63kDa.
    Stained with Coomassie Blue.

Recombinant Human KPNA3 protein (ab114747)参考文献

ab114747 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab114747.
Please use the links above to contact us or submit feedback about this product.