Recombinant人Klotho protein (ab84072)

概述

  • 产品名称Recombinant人Klotho protein
  • 蛋白长度Protein fragment

描述

  • 性质Recombinant
  • 来源CHO cells
  • 氨基酸序列
    • 种属Human
    • 序列EPGDGAQTWA RFSRPPAPEA AGLFQGTFPD GFLWAVGSAA YQTEGGWQQH GKGASIWDTF THHPLAPPGD SRNASLPLGA PSPLQPATGD VASDSYNNVF RDTEALRELG VTHYRFSISW ARVLPNGSAG VPNREGLRYY RRLLERLREL GVQPVVTLYH WDLPQRLQDA YGGWANRALA DHFRDYAELC FRHFGGQVKY WITIDNPYVV AWHGYATGRL APGIRGSPRL GYLVAHNLLL AHAKVWHLYN TSFRPTQGGQ VSIALSSHWI NPRRMTDHSI KECQKSLDFV LGWFAKPVFI DGDYPESMKN NLSSILPDFT ESEKKFIKGT ADFFALCFGP TLSFQLLDPH MKFRQLESPN LRQLLSWIDL EFNHPQIFIV ENGWFVSGTT KRDDAKYMYY LKKFIMETLK AIKLDGVDVI GYTAWSLMDG FEWHRGYSIR RGLFYVDFLS QDKMLLPKSS ALFYQKLIEK NGFPPLPENQ PLEGTFPCDF AWGVVDNYIQ VSQLTKPISS LTKPYH
    • 氨基酸34 to 549

技术指标

Our Abpromise guarantee covers the use of ab84072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Biological Activity : Determined by the dose-dependent stimulation of the proliferation of murine NIH3T3 cells.
  • 应用

    Functional Studies

    SDS-PAGE

  • 内毒素水平< 0.100 Eu/µg
  • 纯度> 95 % SDS-PAGE.
    ab84072 is greater than 98% by SDS-PAGE gel and HPLC analyses.
  • 形式Lyophilised
  • 补充说明Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶Reconstituted ab84072 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20oC. Avoid repeated freeze/thaw cycles.

常规信息

  • 别名
    • Kl
    • KLOT_HUMAN
    • Klotho peptide
  • 功能May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1.
    The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.
  • 组织特异性Present in cortical renal tubules (at protein level). Soluble peptide is present in serum and cerebrospinal fluid. Expressed in kidney, placenta, small intestine and prostate. Down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure kidney.
  • 疾病相关Defects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC) [MIM:211900]. A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement.
  • 序列相似性Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.
  • 结构域Contains 2 glycosyl hydrolase 1 regions. However, the first region lacks the essential Glu active site residue at position 239, and the second one lacks the essential Glu active site residue at position 872.
  • 翻译后修饰N-glycosylated.
  • 细胞定位Secreted and Cell membrane. Isoform 1 shedding leads to a soluble peptide.
  • Information by UniProt

Recombinant human Klotho protein (ab84072)参考文献

ab84072 has not yet been referenced specifically in any publications.

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Leider ist das Klotho-Fragment der Aminosäuren 34-549 das einzige Klotho, das wir verfügbar haben. Unter ab84072 bieten wir die ungetagte Form an, unter ab124316 ist das gleiche Fragment mit einem ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"