The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. A His-tag fusion protein of KIR3DL1 cytoplasmic tail was overexpressed as insoluble protein aggregates (inclusion bodies). This protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer.
The cytoplasmic tail of KIR3DL1, which contains two immunoreceptor tyrosine-based inhibition motifs (ITIMs), mediates inhibitory signal transduction that prevents killer cell-mediated cytotoxicity.
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.