重组人KDM5C / Jarid1C / SMCX蛋白(ab125608)

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概述

描述

  • 性质
    Recombinant
  • 来源
    Baculovirus infected Sf9 cells
  • 氨基酸序列
    • Accession
      P41229
    • 种属
      Human
    • 分子量
      118 kDa including tags
    • 氨基酸
      1 to 671
    • 标签
      GST tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab125608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    SDS-PAGE

  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

常规信息

  • 别名
    • DXS1272E
    • Histone demethylase JARID1C
    • JARID1C
    • JmjC domain containing protein SMCX
    • Jumonji AT rich interactive domain 1C
    • Jumonji, AT rich interactive domain 1C (RBP2 like)
    • Jumonji/ARID domain-containing protein 1C
    • KDM5C
    • KDM5C_HUMAN
    • Lysine (K) specific demethylase 5C
    • Lysine-specific demethylase 5C
    • MRX13
    • MRXJ
    • MRXSCJ
    • MRXSJ
    • Protein SmcX
    • Protein Xe169
    • rbp2 like protein
    • Selected cDNA on X
    • SMCX
    • Smcx homolog X chromosome
    • SmcX protein
    • Smcy homolog X linked
    • XE169
    • Xe169 protein
    see all
  • 功能
    Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements.
  • 组织特异性
    Expressed in all tissues examined. Highest levels found in brain and skeletal muscle.
  • 疾病相关
    Defects in KDM5C are the cause of mental retardation syndromic X-linked JARID1C-related (MRXSJ) [MIM:300534]. MRXSJ is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRXSJ patients manifest mental retardation associated with variable features such as slowly progressive spastic paraplegia, seizures, facial dysmorphism.
  • 序列相似性
    Belongs to the JARID1 histone demethylase family.
    Contains 1 ARID domain.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
    Contains 2 PHD-type zinc fingers.
  • 结构域
    The first PHD-type zinc finger domain recognizes and binds H3-K9Me3.
    Both the JmjC domain and the JmjN domain are required for enzymatic activity.
  • 细胞定位
    Nucleus.
  • Information by UniProt

图片

  • SDS-PAGE - KDM5C / Jarid1C / SMCX protein (Tagged) (ab125608)
    SDS-PAGE - KDM5C / Jarid1C / SMCX protein (Tagged) (ab125608)
    SDS-PAGE analysis of ab125608.

文献

ab125608 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab125608？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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1 Review or Q&A

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Inquiry: Dear Sirs, I would like to test eventual enzymatic activity of your recombinant protein. I would like to know how is it purified from baculovirus infected cells. Is it likely in native form or is it bound to be completely denatured ? Thank you...

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Unfortunately, ab125608will show no activityas the storage buffer is not suitable for this purpose. In addition, it is a fragment, not the full length protein.

I hope this information is helpful to you. Please do not hesitate to cont...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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