Recombinant人KDM1 / LSD1 protein (ab80379)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • 种属
      Human
    • 氨基酸
      158 to 852
    • 标签
      GST tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab80379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    Specific Activity: 0.82 pmole/min/µg. Assay buffer: 50 mM sodium phosphate, pH 7.4, 70 uM dimethylated H3-K4 peptide and 8 ng/ul recombinant KDM1 / LSD1, 30 min at 37oC.
  • 应用

    Functional Studies

    SDS-PAGE

  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 0.05% Tween 20, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Amine oxidase (flavin containing) domain 2
    • Amine oxidase, flavin containing, 2
    • AOF2
    • BHC110
    • BRAF35 HDAC complex protein BHC110
    • BRAF35-HDAC complex protein BHC110
    • BRAF35/HDAC complex, 110-kD subunit
    • CPRF
    • EC1
    • FAD binding protein BRAF35 HDAC complex, 110 kDa subunit
    • Flavin-containing amine oxidase domain-containing protein 2
    • KDM 1
    • KDM1
    • Kdm1a
    • KDM1A_HUMAN
    • KIAA0601
    • LSD 1
    • LSD1
    • Lysine (K) specific demethylase 1
    • Lysine (K) specific demethylase 1A
    • Lysine demethylase 1A
    • Lysine specific histone demethylase 1
    • Lysine specific histone demethylase 1A
    • Lysine-specific demethylase 1
    • Lysine-specific demethylase 1A
    • Lysine-specific histone demethylase 1A
    see all
  • 功能
    Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.
  • 组织特异性
    Ubiquitously expressed.
  • 序列相似性
    Belongs to the flavin monoamine oxidase family.
    Contains 1 SWIRM domain.
  • 结构域
    The SWIRM domain may act as an anchor site for a histone tail.
  • 细胞定位
    Nucleus.
  • Information by UniProt

图片

  • Image showing specific activity of ab80379.
  • 10% SDS-PAGE showing ab80379 at approximately 103kDa (2µg).

文献

ab80379 has not yet been referenced specifically in any publications.

客户评价及客户问答

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1. Both negative controls (Lane1) with two different antibodies are showing already...

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