Recombinant人Kallikrein 6 protein (ab82792)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • 种属
      Human
    • 氨基酸
      17 to 244

技术指标

Our Abpromise guarantee covers the use of ab82792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 形式
    Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.00

  • 复溶
    500ul distilled water for reconstitution then 20mM HEPES, pH 7.0

常规信息

  • 别名
    • Bssp
    • hK 6
    • hK6
    • Kallikrein 6 precursor
    • Kallikrein related peptidase 6
    • Kallikrein-6
    • Kallikrein6
    • KLK 6
    • Klk 7
    • KLK 9
    • Klk29
    • Klk6
    • KLK6_HUMAN
    • Klk7
    • KLK9
    • MGC9355
    • mGK 1
    • mGK1
    • MSP
    • Neurosin
    • Protease M
    • Protease serine 18
    • Protease serine 9
    • PRSS 18
    • PRSS 9
    • PRSS18
    • PRSS9
    • Serine protease 18
    • Serine protease 9
    • SP 59
    • SP59
    • Tissue kallikrein
    • TK
    • Zyme
    see all
  • 功能
    Serine protease which exhibits a preference for Arg over Lys in the substrate P1 position and for Ser or Pro in the P2 position. Shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein and extracellular matrix proteins such as fibronectin, laminin, vitronectin and collagen. Degrades alpha-synuclein and prevents its polymerization, indicating that it may be involved in the pathogenesis of Parkinson disease and other synucleinopathies. May be involved in regulation of axon outgrowth following spinal cord injury. Tumor cells treated with a neutralizing KLK6 antibody migrate less than control cells, suggesting a role in invasion and metastasis.
  • 组织特异性
    In fluids, highest levels found in milk of lactating women followed by cerebrospinal fluid, nipple aspirate fluid and breast cyst fluid. Also found in serum, seminal plasma and some amniotic fluids and breast tumor cytosolic extracts. Not detected in urine. At the tissue level, highest concentrations found in glandular tissues such as salivary glands followed by lung, colon, fallopian tube, placenta, breast, pituitary and kidney. Not detected in skin, spleen, bone, thyroid, heart, ureter, liver, muscle, endometrium, testis, pancreas, seminal vesicle, ovary, adrenals and prostate. In brain, detected in gray matter neurons (at protein level). Colocalizes with pathological inclusions such as Lewy bodies and glial cytoplasmic inclusions. Overexpressed in primary breast tumors but not expressed in metastatic tumors.
  • 序列相似性
    Belongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • 翻译后修饰
    Inactivated by autolytic cleavage after Arg-80.
  • 细胞定位
    Secreted. Nucleus > nucleolus. Cytoplasm. Mitochondrion. Microsome. In brain, detected in the nucleus of glial cells and in the nucleus and cytoplasm of neurons. Detected in the mitochondrial and microsomal fractions of HEK-293 cells and released into the cytoplasm following cell stress.
  • Information by UniProt

图片

  • SDS-PAGE showing ab82792 at approximately 51.8kDa.

文献

ab82792 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Other

Review text: Ab82792 is a recombinant full length human Kallikrein 6, which was loaded and co-electrophoresed along with the Western C marker (BioRad) for 50 min and then the gel was stained with Coomassie Blue Staining and the membrane was stained with Ponceau S in order to visualise this protein on the gel and the membrane.
Sample: Human Recombinant protein

Primary antibody (in addition to 'Kallikrein 6 protein (Tagged)')
Primary antibody: None used

Secondary antibody
Secondary antibody: None used
Username

Abcam user community

Verified customer

提交于 Oct 23 2012

Thank you for contacting us last week in regards to the Kallikrein 6 protein (ab82792).

I am sorry for the confusion caused by the datasheet for this product. I can now explain how the protein should be reconstituted. You need to add 500 &mic...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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