Recombinant人JNK2 protein (ab119726)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • AccessionP45984
    • 种属Human
    • 分子量70 kDa including tags
    • 氨基酸1 to 424

技术指标

Our Abpromise guarantee covers the use of ab119726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性ab119726 specific activity was determined to be 13 nmol/min/mg.
  • 应用

    Western blot

    Functional Studies

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.

  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 25% Glycerol, 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.003% DTT, 0.002% PMSF, 0.003% EDTA

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • c Jun kinase 2
    • C Jun N terminal kinase 2
    • c-Jun N-terminal kinase 2
    • JNK 55
    • JNK-55
    • JNK2
    • JNK2 alpha
    • JNK2 beta
    • JNK2A
    • JNK2alpha
    • JNK2B
    • JNK2BETA
    • Jun kinase
    • MAP kinase 9
    • MAPK 9
    • Mapk9
    • Mitogen activated protein kinase 9
    • Mitogen-activated protein kinase 9
    • MK09_HUMAN
    • P54a
    • p54aSAPK
    • PRKM9
    • SAPK
    • SAPK alpha
    • SAPK1a
    • Stress activated protein kinase 1a
    • Stress-activated protein kinase JNK2
    see all
  • 功能Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.
    JNK2 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it.
  • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • 结构域The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • 翻译后修饰Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. Autophosphorylated in vitro.
  • Information by UniProt

Recombinant human JNK2 protein 图像

  • ab119726 specific activity was determined to be 13 nmol/min/mg.
  • SDS Page analysis of ab119726

Recombinant human JNK2 protein (ab119726)参考文献

ab119726 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam with your questions pertaining to ab119726. I have been in contact with the laboratory about the questions that you have raised. The tag used in this preparation is GST. ab1198726 may be used in conjunction wi...

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