重组人JNK2蛋白(ab119726)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
描述
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产品名称
重组人JNK2蛋白
参阅全部 JNK2 蛋白酶 -
生物活性
ab119726 specific activity was determined to be 13 nmol/min/mg.
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纯度
> 95 % SDS-PAGE.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
70 kDa including tags -
氨基酸
1 to 382 -
标签
GST tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab119726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
Functional Studies
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.003% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- c Jun kinase 2
- C Jun N terminal kinase 2
- c-Jun N-terminal kinase 2
see all -
功能
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.
JNK2 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
结构域
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻译后修饰
Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. Autophosphorylated in vitro. - Information by UniProt
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab119726 尚未被引用在任何文献中。