Recombinant人JNK1 (mutated K55 M) protein (ab95248)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 氨基酸2 to 383

技术指标

Our Abpromise guarantee covers the use of ab95248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Functional Studies

    SDS-PAGE

  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0

常规信息

  • 别名
    • AI849689
    • c Jun N terminal kinase 1
    • C-JUN kinase 1
    • c-Jun N-terminal kinase 1
    • EC 2.7.11.24
    • JNK
    • JNK 1
    • JNK-46
    • JNK1A2
    • JNK21B1/2
    • MAP kinase 8
    • MAPK 8
    • mapk8
    • Mitogen activated protein kinase 8
    • Mitogen-activated protein kinase 8
    • MK08_HUMAN
    • p54 gamma
    • Prkm8
    • Protein kinase JNK1
    • Protein kinase, mitogen-activated, 8
    • SAPK 1
    • SAPK gamma
    • SAPK1
    • Stress-activated protein kinase 1
    • Stress-activated protein kinase JNK1
    see all
  • 功能Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
    JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
  • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • 结构域The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • 翻译后修饰Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
  • Information by UniProt

Recombinant Human JNK1 (mutated K55 M) protein 图像

  • ab95248 tested by SDS-PAGE on a 10% gel followed by staining with coomassie blue. Lane 1; ab95248 at 14 µg. Lane 2; protein molecular weight markers. The predicted molecular weight is 71 kDa and ab95248 is assessed as being >85% pure.

Recombinant Human JNK1 (mutated K55 M) protein (ab95248)参考文献

This product has been referenced in:
  • Krishna SN  et al. A fluorescence-based thermal shift assay identifies inhibitors of mitogen activated protein kinase kinase 4. PLoS One 8:e81504 (2013). Read more (PubMed: 24339940) »

See 1 Publication for this product

Product Wall

I will send a vial of that then.

But first, I have one more obvious question which you may have already answered in our conversation: Do you have evidence that the protein ab95248 is intact, for instance a Coomassie stain of the gel? If not,...

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Given that we do not have other lots of ab10664, would you be interested in this JNK1/JNK2 antibody, ab85139?

Click here (or use the following: http://www.abcam.com/index.html?datasheet=85139).

If you want to avoid reactivity with...

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Thank you for calling Abcam. I have heard back from the lab about ab95248, JUNK1 protein. They say that it is ok to remove the TRIS and DTT and store in PBS.

This protein is not phosphorylated, however it can be as we know if can be used as a kinase substrate.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"