Recombinant人JAK3 protein (ab89982)

概述

描述

  • 性质
    Recombinant
  • 来源
    Baculovirus
  • 氨基酸序列
    • 种属
      Human
    • 氨基酸
      781 to 1124

技术指标

Our Abpromise guarantee covers the use of ab89982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    Specific Activity: 118 pmol/min/µg. Assay conditions: 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate at room temperature for 1 hour.
  • 应用

    Functional Studies

    SDS-PAGE

  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on Dry Ice. Store at -80°C.

    Preservative: None
    Constituents: 20% Glycerol, 0.05% Tween 20, 25mM Tris HCl, 100mM Sodium chloride, 1mM DTT, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • EC 2.7.10.2
    • JAK 3
    • JAK L
    • JAK-3
    • Jak3
    • JAK3 HUMAN
    • JAK3_HUMAN
    • JAKL
    • Janus kinase 3
    • Janus kinase 3 (a protein tyrosine kinase, leukocyte)
    • Janus Kinase3
    • L JAK
    • L-JAK
    • Leukocyte janus kinase
    • LJAK
    • Protein tyrosine kinase leukocyte
    • Tyrosine protein kinase JAK3
    • Tyrosine-protein kinase JAK3
    see all
  • 功能
    Tyrosine kinase of the non-receptor type, involved in the interleukin-2 and interleukin-4 signaling pathway. Phosphorylates STAT6, IRS1, IRS2 and PI3K.
  • 组织特异性
    In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B-form is detected in cells both of hematopoietic and epithelial origins.
  • 疾病相关
    Defects in JAK3 are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-negative (T(-)B(+)NK(-) SCID) [MIM:600802]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
  • 序列相似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
  • 结构域
    Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
  • 翻译后修饰
    Tyrosine phosphorylated in response to IL-2 and IL-4.
  • 细胞定位
    Endomembrane system. Wholly intracellular, possibly membrane associated.
  • Information by UniProt

图片

  • Image showing specific activity of ab89982.
  • 10% SDS-PAGE showing ab89982 at approximately 65kDa (5µg).

文献

ab89982 has not yet been referenced specifically in any publications.

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