Recombinant人JAK3 protein (ab89756)

概述

描述

  • 性质
    Recombinant
  • 来源
    Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属
      Human
    • 氨基酸
      781 to 1124

相关产品

技术指标

Our Abpromise guarantee covers the use of ab89756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    The Specific activity of ab89756 was determined to be 91 nmol/min/mg.
  • 应用

    SDS-PAGE

    Western blot

    Functional Studies

  • 形式
    Liquid
  • 补充说明

    ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • EC 2.7.10.2
    • JAK 3
    • JAK L
    • JAK-3
    • Jak3
    • JAK3 HUMAN
    • JAK3_HUMAN
    • JAKL
    • Janus kinase 3
    • Janus kinase 3 (a protein tyrosine kinase, leukocyte)
    • Janus Kinase3
    • L JAK
    • L-JAK
    • Leukocyte janus kinase
    • LJAK
    • Protein tyrosine kinase leukocyte
    • Tyrosine protein kinase JAK3
    • Tyrosine-protein kinase JAK3
    see all
  • 功能
    Tyrosine kinase of the non-receptor type, involved in the interleukin-2 and interleukin-4 signaling pathway. Phosphorylates STAT6, IRS1, IRS2 and PI3K.
  • 组织特异性
    In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B-form is detected in cells both of hematopoietic and epithelial origins.
  • 疾病相关
    Defects in JAK3 are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-negative (T(-)B(+)NK(-) SCID) [MIM:600802]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
  • 序列相似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
  • 结构域
    Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
  • 翻译后修饰
    Tyrosine phosphorylated in response to IL-2 and IL-4.
  • 细胞定位
    Endomembrane system. Wholly intracellular, possibly membrane associated.
  • Information by UniProt

图片

  • Kinase Assay demonstrating specific activity of ab89756.
  • SDS-PAGE showing ab89756 at approximately 64kDa.

文献

ab89756 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab89756.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册