Recombinant人Isocitrate dehydrogenase protein (ab113858)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionO75874
    • 种属Human
    • 序列MGSSHHHHHHSSGLVPRGSHMSKKISGGSVVEMQGDEMTRIIWELIKEKL IFPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKHNVGVKCATITPDE KRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVSGWVKPIII GRHAYGDQYRATDFVVPGPGKVEITYTPSDGTQKVTYLVHNFEEGGGVAM GMYNQDKSIEDFAHSSFQMALSKGWPLYLSTKNTILKKYDGRFKDIFQEI YDKQYKSQFEAQKIWYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDS VAQGYGSLGMMTSVLVCPDGKTVEAEAAHGTVTRHYRMYQKGQETSTNPI ASIFAWTRGLAHRAKLDNNKELAFFANALEEVSIETIEAGFMTKDLAACI KGLPNVQRSDYLNTFEFMDKLGENLKIKLAQAKL
    • 分子量49 kDa including tags
    • 氨基酸1 to 414
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab113858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific activity: > 0.7 units/ml. One unit will convert 1.0 umole of isocitrate to alpha-ketoglutarate per minute at pH7.5 at 25C.
  • 应用

    Mass Spectrometry

    SDS-PAGE

  • 质谱法
    MALDI-TOF
  • 纯度> 95 % SDS-PAGE.
    ab113858 was purified using conventional chromatography.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 20% Glycerol, 0.58% Sodium chloride, 0.02% DTT, 0.002% PMSF

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Cytosolic NADP isocitrate dehydrogenase
    • Cytosolic NADP-isocitrate dehydrogenase
    • Epididymis luminal protein 216
    • Epididymis secretory protein Li 26
    • HEL-216
    • HEL-S-26
    • ICDH
    • IDCD
    • IDH
    • IDH1
    • IDHC_HUMAN
    • IDP
    • IDPC
    • Isocitrate dehydrogenase [NADP] cytoplasmic
    • Isocitrate dehydrogenase 1 (NADP+) soluble
    • NADP dependent isocitrate dehydrogenase cytosolic
    • NADP dependent isocitrate dehydrogenase peroxisomal
    • NADP(+)-specific ICDH
    • Oxalosuccinate decarboxylase
    • PICD
    see all
  • 疾病相关Glioma
    Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate.
  • 序列相似性Belongs to the isocitrate and isopropylmalate dehydrogenases family.
  • 翻译后修饰Acetylation at Lys-374 dramatically reduces catalytic activity.
  • 细胞定位Cytoplasm. Peroxisome.
  • Information by UniProt

Recombinant human Isocitrate dehydrogenase protein 图像

  • 15% SDS-PAGE analysis of 3 µg ab113858.

Recombinant human Isocitrate dehydrogenase protein (ab113858)参考文献

ab113858 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

I was in touch with the supplying lab and they weren't sure why the higher band was being detected.They verified this protein with 15% SDS-PAGE and hadn't identified anyimpurities above 70kDa.
They wondered ifth...

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Thank you for your inquiry. I am sorry to hear about the purity problem with ab113858.

Do you think you could resend the gel image? Unfortunately it didn't go through. Usually .jpg, .ppt, .doc, or .pdf transfer well.

Can you tell me...

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Thank you for contacting Abcam.

The bioactivity has just been tested and the information os below:

Specific activity: > 0.7 units/ml. One unit will convert 1.0 umole of isocitrate to alpha-ketoglutarate per minute at pH7.5 at 25C...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"