Recombinant人Interferon gamma protein (ab9659)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionQ14609
    • 种属Human
    • 序列MQDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKR KRSQMLFQGR RASQ
    • 分子量17 kDa
    • 氨基酸1 to 144

技术指标

Our Abpromise guarantee covers the use of ab9659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性

    The ED50 determined by a cytotoxicity assay using HT-29 cells is ≤0.05 ng/mL, corresponding to a specific activity of ≥ 2 x 107 units/mg.

     

  • 应用

    Functional Studies

    SDS-PAGE

  • 内毒素水平< 1.000 Eu/µg
  • 纯度> 97 % SDS-PAGE.
    Sterile filtered. Greater than 97% by SDS-PAGE and HPLC analyses.
  • 形式Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    n/a

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶Reconstitute in 100ul PBS, pH 8.0 to a concentration of 1.0 mg/ml. Note: further dilutions should be made into RPMI + 10% FCS or 5% HAS and stored at 4 degrees C for 1 week or -20 degrees C for future use.

常规信息

  • 别名
    • IFG
    • IFI
    • IFN gamma
    • IFN, immune
    • IFN-gamma
    • IFNG
    • IFNG_HUMAN
    • Immune interferon
    • Interferon gamma
    see all
  • 功能Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • 组织特异性Released primarily from activated T lymphocytes.
  • 疾病相关In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • 序列相似性Belongs to the type II (or gamma) interferon family.
  • 翻译后修饰Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • 细胞定位Secreted.
  • Information by UniProt

Recombinant human Interferon gamma protein (ab9659)参考文献

This product has been referenced in:
  • Wu Z  et al. Oxidative stress modulates complement factor H expression in retinal pigmented epithelial cells by acetylation of FOXO3. J Biol Chem 282:22414-25 (2007). Read more (PubMed: 17558024) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"