Recombinant人IL5 protein (ab83941)

概述

描述

  • 性质Recombinant
  • 来源HEK 293 cells
  • 氨基酸序列
    • 种属Human
    • 序列Theoretical sequence: IPTEIPTSALVKETLALLSTHRTLLIANETLRIPVPVHK NHQLCTEEIF QGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCG EERRRVNQFLD YLQEFLGVMNTEWIIES

技术指标

Our Abpromise guarantee covers the use of ab83941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.

  • 形式Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C.

    Preservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin

  • 复溶It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

常规信息

  • 别名
    • B-cell differentiation factor I
    • Colony stimulating factor
    • EDF
    • Eosinophil differentiation factor
    • IL-5
    • IL5
    • IL5_HUMAN
    • Interleukin 5
    • Interleukin 5 (colony stimulating factor, eosinophil)
    • Interleukin-5
    • T Cell Replacing Factor
    • T-cell replacing factor
    • TRF
    see all
  • 功能Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
  • 序列相似性Belongs to the IL-5 family.
  • 细胞定位Secreted.
  • Information by UniProt

Recombinant Human IL5 protein 图像

  • Lane 1 – MW markers; Lane 2 – ab83941; Lane 3 – ab83941 treated with PNGase F to remove potential N-linked glycans; Lane 4 – ab83941 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. 10 ug protein loaded per lane. Deep Purple stained.
    Appearance of two bands after treatment with PNGase F indicates presence of N-linked glycans. Subsequent drop in MW of top band after treatment with glycosidase cocktail indicates presence of O-linked glycans.

    Additional bands in lane 3 and lane 4 are glycosidase enzymes.
  • Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
    The triangle indicates theoretical pI and MW of theprotein.

Recombinant Human IL5 protein (ab83941)参考文献

ab83941 has not yet been referenced specifically in any publications.

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