The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Shipped at 4°C. Store at +4°C.
Constituents: 10% Trehalose, 1% Human serum albumin
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Lane 1 – MW markers; Lane 2 – ab83941; Lane 3 – ab83941 treated with PNGase F to remove potential N-linked glycans; Lane 4 – ab83941 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. 10 ug protein loaded per lane. Deep Purple stained. Appearance of two bands after treatment with PNGase F indicates presence of N-linked glycans. Subsequent drop in MW of top band after treatment with glycosidase cocktail indicates presence of O-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.
Functional Studies - IL5 protein (ab83941)
Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification. The triangle indicates theoretical pI and MW of theprotein.
Recombinant Human IL5 protein (ab83941)参考文献
has not yet been referenced specifically in any publications.