The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 10% Trehalose, 1% Human serum albumin, PBS
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
High affinity receptor for interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor.
Belongs to the type I cytokine receptor family. Type 4 subfamily. Contains 2 fibronectin type-III domains.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif is required for JAK interaction and/or activation.
Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified Human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational
The triangle indicates the theoretical MW and pI of the protein.
SDS-PAGE - IL3RB– Fc Chimera (ab83919)
1D SDS-PAGE of ab83919 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab83919
Lane 2: ab83919 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab83919 treated with a glycosidase cocktail to
remove potential N- and O-linked glycans.
Approximately 5 μg of protein was loaded per lane; Gel was stained using Deep Purple™.
Recombinant Human IL3RB protein (Fc Chimera) (ab83919)参考文献
has not yet been referenced specifically in any publications.