重组人IL3RA/CD123蛋白(Fc Chimera) (ab88358)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: Fc tag C-Terminus
- Suitable for: SDS-PAGE
描述
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产品名称
重组人IL3RA/CD123蛋白(Fc Chimera)
参阅全部 IL3RA/CD123 蛋白酶 -
纯度
> 95 % SDS-PAGE.
Determined by SDS-PAGE and visualized by silver stain. Purified by native chromatography methods without the use of molecular tags. -
内毒素水平
< 1.000 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
TKEDPNPPITNLRMKAKAQQLTWDLNRNVTDIECVKDADYSMPAVN NS YCQFGAISLCEVTNYTVRVANPPFSTWILFPENSGKPWAGAENL TCWI HDVDFLSCSWAVGPGAPADVQYDLYLNVANRRQQYECLHYKT DAQGTR IGCRFDDISRLSSGSQSSHILVRGRSAAFGIPCTDKFVVF SQIEILTP PNMTAKCNKTHSFMHWKMRSHFNRKFRYELQIQKRMQP VITEQVRDRT SFQLLNPGTYTVQIRARERVYEFLSAWSTPQRFECD QEEGANTRGGRV DGIQWIPKVDKKVEPKSCDKTHTCPPCPAPELLG GPSVFLFPPKPKDT LMISRTPEVTCVVVDVSHEDPEVKFNWYVDGV EVHNAKTKPREEQYNS TYRVVSVLTVLHQDWLNGKEYKCRVSNKAL PAPIEKTISKAKGQPREP QVYTLPPSRDELTKNQVSLTCLVKGFYP SDIAVEWESNGQPENNYKTT PPVLDSDGSFFLYSKLTVDKSRWQQG NVFSCSVMHEALHNHYTQKSLS LSPGK -
氨基酸
19 to 302 -
标签
Fc tag C-Terminus -
额外的序列信息
DNA sequence encoding the signal peptide and extracellular domain of human IL3R alpha (aa 1-18) was fused to the Fc region of human IgG1 (aa 93-330). Protein expressed in modified human 293 cells.
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技术指标
Our Abpromise guarantee covers the use of ab88358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Lyophilized -
补充说明
Previously labelled as IL3RA.
Molecular Mass: ab88358 migrates as a broad band between 85 and 100 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified protein that has a predicted molecular mass of 60.6 kDa.
ab88358 consists of 25-40% carbohydrate by weight. pI: ab88358 separates into a number of isoforms with a pI between 6.2 and 9.0 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified protein that has a predicted pI of 8.43. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 1% Human serum albumin, 10% Trehalose
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复溶It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
常规信息
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别名
- CD123
- CD123 antigen
- hIL 3Ra
see all -
功能
This is a receptor for interleukin-3. -
序列相似性
Belongs to the type I cytokine receptor family. Type 5 subfamily. -
结构域
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
细胞定位
Membrane. - Information by UniProt
图片
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1D SDS-PAGE of ab88358 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab88358
Lane 2: ab88358 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab88358 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Drop in MWt after treatment with PNGase F indicates presence of N-linked glycans. Subsequent drop in MWt after treatment with glycosidase cocktail indicates presence of O-linked glycans. Faint bands in lane 2 and lane 3 are glycosidase enzymes. -
A sample of ab88358 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. Spot train indicates presence of multiple isoforms of ab88358.
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Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
The triangle indicates the theoretical MWt and pI of the protein.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab88358 被引用在 1 文献中.
- Riberdy JM et al. The Art and Science of Selecting a CD123-Specific Chimeric Antigen Receptor for Clinical Testing. Mol Ther Methods Clin Dev 18:571-581 (2020). PubMed: 32775492