重组人IL31蛋白(ab106619)

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概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • Accession
      Q6EBC2
    • 种属
      Human
    • 序列
      SHTLPVRLLR PSDDVQKIVE ELQSLSKMLL KDVEEEKGVL VSQNYTLPCL SPDAQPPNNI HSPAIRAYLK TIRQLDNKSV IDEIIEHLDK LIFQDAPETN ISVPTDTHEC KRFILTISQQ FSECMDLALK SLTSGAQQAT T
    • 分子量
      16 kDa
    • 氨基酸
      24 to 164

技术指标

Our Abpromise guarantee covers the use of ab106619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    Measured by its ability to induce STAT3 activation in Human U87 cells.
  • 应用

    SDS-PAGE

    HPLC

    ELISA

    Western blot

  • 内毒素水平
    < 0.100 Eu/µg
  • 纯度
    > 98 % SDS-PAGE.
    Greater than 98% by SDS-PAGE gel and HPLC analyses
  • 形式
    Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶
    The lyophilised product should be stored at -20oC till use. Once the product has been reconstituted according to the CoA, this can be diluted further in a chosen aqueous buffer containing a carrier protein, such as 0.1% BSA and stored in working aliquots at -20oC for at least 3 months

常规信息

  • 别名
    • IL 31
    • IL-31
    • IL31
    • IL31_HUMAN
    • Interleukin 31
    • Interleukin-31
    see all
  • 功能
    Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity.
  • 组织特异性
    Detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea.
  • 细胞定位
    Secreted.
  • Information by UniProt

图片

  • Western blot - IL31 protein (ab106619)
    Western blot - IL31 protein (ab106619)Image courtesy of an anonymous Abreview.
    Whole cell lysate prepared from human eosinophil granulocytes was loaded at 500000 cells.
    5-16% gel run under reducing, denaturing conditions.
    Blocking step performed using 5% BSA for 2 hours at 25°C.
    ab106619 used at a 1/200 dilution.
    Primary antibody used at a 1/100 dilution.
    Secondary antibody was an HRP conjugated goat anti-rabbit IgG, used at a 1/200 dilution.
    The positive control was stimulated with IL-3 (10ng/ml) + IL-5 (10ng/ml) + GM-CSF (10ng/ml) for 30 minutes.

    See Abreview

文献

ab106619 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab106619？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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ELISA Abreview for IL31 protein

 Good
Abreviews
Application
ELISA

Sample: Human Serum (human serum samples)
Specification: human serum samples
Blocking step: BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Type: Direct

Other product details
Dilution: 1/30000
Incubation time: 2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS with 0,1%BSA and 0,05%Tween20

Primary antibody (in addition to 'IL31 protein')
Name: Non-Abcam antibody was used: monoclonal anti IL-31
Host species: Mouse
Clonality: Monoclonal
Dilution: 1/250

Secondary antibody
Name: Non-Abcam antibody was used: monoclonal anti IL31
Host species: Mouse
Clonality: Monoclonal
Conjugation: Biotin
Dilution: 1/2000

Additional data
Additional Notes: IL-31 used as standard
range: 3-300pg/ml
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提交于 Jan 21 2013

Western blot Abreview for IL31 protein

 Good
Abreviews
Application
Western blot

Sample: Human Cell lysate - whole cell (eosinophil granulocyte)
Loading amount: 500000 cells
Specification: eosinophil granulocyte
Gel Running Conditions: Reduced Denaturing (5-16%)
Blocking step: BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details
Dilution: 1/200
Incubation time: 14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA / TBS

Primary antibody (in addition to 'IL31 protein')
Dilution: 1/100

Secondary antibody
Name: Non-Abcam antibody was used: anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/2000

Detection
Detection method: Chemiluminescence
Exposure: 45 minute(s) and 0 second(s)
Bands: Specific: 18 kDa Non-specific: - kDa
Positive control: stimulation with IL-3 (10ng/ml) + IL-5 (10ng/ml) + GM-CSF (10ng/ml) for 30 minutes
Negative control: non stimulated cells
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提交于 Dec 07 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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