Recombinant人IL2 Receptor gamma protein (Fc Chimera) (ab84065)

概述

描述

  • 性质Recombinant
  • 来源HEK 293 cells
  • 氨基酸序列
    • AccessionP31785
    • 种属Human
    • 序列Theoretical Sequence: LNTTILTPNGNEDTTADFFLTTMPTDSLSVSTLPLPEVQ CFVFNVEYM NCTWNSSSEPQPTNLTLHYWYKNSDNDKVQKCSHYLFSE EITSGCQLQ KKEIHLYQTFVVQLQDPREPRRQATQMLKLQNLVIPWAP ENLTLHKLS ESQLELNWNNRFLNHCLEHLVQYRTDWDHSWTEQSVDYR HKFSLPSVD GQKRYTFRVRSRFNPLCGSAQHWSEWSHPIHWGSNTSKE NPFLFAWIP KVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPK DTLMISRTP EVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQY NSTYRVVSV LTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPR EPQVYTLPP SRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYK TTPPVLDSD GSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKS LSLSPGK
    • 氨基酸23 to 259
    • 额外的序列信息DNA sequence encoding the signal peptide and extracellular domain of human IL 2R gamma (aa 1-259) was fused to the Fc region of human IgG1 (aa 93-330). Protein expressed in modified human 293 cells.

技术指标

Our Abpromise guarantee covers the use of ab84065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.

  • 形式Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin

  • 复溶It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

常规信息

  • 别名
    • CD132
    • CD132 antigen
    • CIDX
    • common cytokine receptor gamma chain
    • Cytokine receptor common subunit gamma
    • Gamma C
    • gamma(c)
    • gammaC
    • IL-2 receptor subunit gamma
    • IL-2R gamma chain
    • IL-2R subunit gamma
    • IL-2RG
    • Il2rg
    • IL2RG_HUMAN
    • IMD4
    • interleukin 2 receptor, gamma
    • Interleukin-2 receptor subunit gamma
    • p64
    • SCIDX
    • SCIDX1
    see all
  • 功能Common subunit for the receptors for a variety of interleukins.
  • 疾病相关Defects in IL2RG are the cause of severe combined immunodeficiency X-linked T-cell-negative/B-cell-positive/NK-cell-negative (XSCID) [MIM:300400]; also known as agammaglobulinemia Swiss type. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Defects in IL2RG are the cause of X-linked combined immunodeficiency (XCID) [MIM:312863]. XCID is a less severe form of X-linked immunodeficiency with a less severe degree of deficiency in cellular and humoral immunity than that seen in XSCID.
  • 序列相似性Belongs to the type I cytokine receptor family. Type 5 subfamily.
    Contains 1 fibronectin type-III domain.
  • 结构域The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • 细胞定位Membrane.
  • Information by UniProt

Recombinant Human IL2 Receptor gamma protein (Fc Chimera) 图像

  • 1D SDS-PAGE of ab84065 before and after treatment with glycosidases to remove oligosaccharides.
    Lane 1: ab84065
    Lane 2: ab84065 treated with PNGase F to remove potential N-linked glycans
    Lane 3: ab84065 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
    10 µg protein loaded per lane; Deep Purple™ stained.

    Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Subsequent drop in MW after treatment with glycosidase cocktail indicates presence of O-linked glycans. Faint bands in lane 2 and lane 3 are glycosidase enzymes.
  • A sample of ab84065 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel.
    40 µg protein loaded per lane; Deep Purple™ stained. Spot train indicates presence of multiple isoforms. Spots within the spot train were cut from the gel and identified as IL2 Receptor gamma (Fc Chimera) by protein mass fingerprinting.
  • Densitometry of protein isoforms visualised by 2-DE.
    The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
    The triangle indicates the theoretical MW and pI of the protein.

Recombinant Human IL2 Receptor gamma protein (Fc Chimera) (ab84065)参考文献

ab84065 has not yet been referenced specifically in any publications.

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