Recombinant人IL2 Receptor alpha protein (Fc Chimera) (ab84071)

概述

描述

  • 性质
    Recombinant
  • 来源
    HEK 293 cells
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 序列
      Theoretical Sequence: ELCDDDPPEIPHATFKAMAYKEGTMLNCECKRGFRRIKSGSLYMLCTG NSSHSSWDNQCQCTSSATRNTTKQVTPQPEEQKERKTTEMQSPMQPVD QASLPGHCREPPPWENEATERIYHFVVGQMVYYQCVQGYRALHRGPAE SVCKMTHGKTRWTQPQLICTGEMETSQFPGEEKPQASPEGRPESETSC LVTTTDFQIQTEMAATMETSIFTTGIPKVDKKVEPKSCDKTHTCPPCP APELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWY VDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNK ALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYP SDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNV FSCSVMHEALHNHYTQKSLSLSPGK
    • 氨基酸
      22 to 237

技术指标

Our Abpromise guarantee covers the use of ab84071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 纯度
    > 95 % SDS-PAGE.

  • 形式
    Lyophilised
  • 补充说明
    ab84071 bound to protein A sepharose beads is able to pull down its ligand, IL2.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶
    It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

常规信息

  • 别名
    • Interleukin 2 receptor alpha chain
    • CD25
    • CD25 antigen
    • IDDM10
    • IL 2 receptor alpha subunit
    • IL-2 receptor subunit alpha
    • IL-2-RA
    • IL-2R subunit alpha
    • IL2 RA
    • IL2-RA
    • IL2R
    • IL2R, alpha chain
    • IL2RA
    • IL2RA_HUMAN
    • IMD41
    • Interleukin 2 receptor
    • Interleukin 2 receptor alpha
    • Interleukin-2 receptor subunit alpha
    • p55
    • t-cell growth factor receptor
    • TAC
    • TAC antigen
    • TCGFR
    see all
  • 功能
    Receptor for interleukin-2.
  • 疾病相关
    Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
  • 序列相似性
    Contains 2 Sushi (CCP/SCR) domains.
  • 细胞定位
    Membrane.
  • Information by UniProt

图片

  • 1D SDS-PAGE of ab84071 before and after treatment with glycosidases to remove oligosaccharides.
    Lane 1: ab84071
    Lane 2: ab84071 treated with PNGase F to remove potential N-linked glycans
    Lane 3: ab84071 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
    10 µg protein loaded per lane; Deep Purple™ stained.

    Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Subsequent tightening of band after treatment with glycosidase cocktail suggests presence of O-linked glycans. Additional bands in lane 2 and lane 3 are glycosidase enzymes.
  • A sample of ab84071 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel.
    40µg protein loaded per lane; Deep Purple™ stained. Spot train indicates presence of multiple isoforms.
    Spots within the spot train were cut from the gel and identified as IL2 Receptor alpha (Fc Chimera) by protein mass fingerprinting.
  • Densitometry of protein isoforms visualised by 2-DE.
    The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
    The triangle indicates the theoretical MW and pI of the protein.

文献

ab84071 has not yet been referenced specifically in any publications.

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