Recombinant人IL1RA protein (ab83761)

概述

描述

  • 性质Recombinant
  • 来源HEK 293 cells
  • 氨基酸序列
    • 种属Human
    • 序列Theoretical Sequence: RPSGRKSSKMQAFRIWDVNQKTFYLRNNQLVAGYLQGPNVNLEEKIDV VPIEPHALFLGIHGGKMCLSCVKSGDETRLQLEAVNITDLSENRKQDK RFAFIRSDSGPTTSFESAACPGWFLCTAMEADQPVSLTNMPDEGVMVT KFYFQEDE

技术指标

Our Abpromise guarantee covers the use of ab83761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Activity: The ED50 of IL1RA is typically 30-100 ng/ml as measured by its ability to inhibit IL1 mediated proliferation using the murine D10S cell line.
  • 应用

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.

  • 形式Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

常规信息

  • 别名
    • DIRA
    • ICIL 1RA
    • ICIL-1RA
    • ICIL1RA
    • IL-1ra
    • IL-1ra3
    • IL-1RN
    • IL1 inhibitor
    • IL1F3
    • IL1RA
    • IL1RA_HUMAN
    • IL1RN
    • IL1RN (IL1F3)
    • Interleukin 1 receptor antagonist
    • Interleukin-1 receptor antagonist protein
    • Intracellular IL 1 receptor antagonist type II
    • Intracellular interleukin 1 receptor antagonist (icIL 1ra)
    • IRAP
    • MGC10430
    • MVCD4
    • Type II interleukin 1 receptor antagonist
    see all
  • 功能Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
  • 组织特异性The intracellular form of IL1RN is predominantly expressed in epithelial cells.
  • 疾病相关Microvascular complications of diabetes 4
    Interleukin 1 receptor antagonist deficiency
  • 细胞定位Cytoplasm and Secreted.
  • Information by UniProt

Recombinant human IL1RA protein 图像

  • Densitometry of protein isoforms visualised by 2-DE.
    The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
    The triangle indicates the theoretical MW and pI of the protein.
  • 1D SDS-PAGE of ab83761 before and after treatment with glycosidases to remove oligosaccharides.
    Lane 1: ab83761
    Lane 2: ab83761 treated with PNGase F to remove potential N-linked glycans.
    Lane 3: ab83761 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
    10 µg protein loaded per lane.
    Drop in MW after treatment with PNGase F indicates the presence of N-linked glycans. Faint bands in lane 3 and lane 4 are glycosidase enzymes.

Recombinant human IL1RA protein (ab83761)参考文献

ab83761 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab83761.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"