The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The ED50 of IL1RA is typically 30-100 ng/ml as measured by its ability to inhibit IL1 mediated proliferation using the murine D10S cell line.
Concentration information loading...
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 10% Trehalose, 1% Human serum albumin
This product is an active protein and may elicit a biological response in vivo, handle with caution.
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
The intracellular form of IL1RN is predominantly expressed in epithelial cells.
Microvascular complications of diabetes 4 Interleukin 1 receptor antagonist deficiency
Functional Studies - IL1RA antagonist protein (Active) (ab83761)
Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational
The triangle indicates the theoretical MW and pI of the protein.
SDS-PAGE - IL1RA antagonist protein (Active) (ab83761)
1D SDS-PAGE of ab83761 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab83761
Lane 2: ab83761 treated with PNGase F to remove potential N-linked glycans.
Lane 3: ab83761 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
10 µg protein loaded per lane.
Drop in MW after treatment with PNGase F indicates the presence of N-linked glycans.
Faint bands in lane 3 and lane 4 are glycosidase enzymes.
Recombinant human IL1RA protein (ab83761)参考文献
has not yet been referenced specifically in any publications.