Recombinant人IL16 protein (ab84082)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列SAASASAASD VSVESTAEAT VCTVTLEKMS AGLGFSLEGG KGSLHGDKPL TINRIFKGAA SEQSETVQPG DEILQLGGTA MQGLTRFEAW NIIKALPDGP VTIVIRRKSL QSKETTAAGD S

技术指标

Our Abpromise guarantee covers the use of ab84082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Biological Activity: Determined by its ability ot chemoattract Human CD4+ T-Lymphocytes using a concentration range of 50.0-100.0 ng/ml.
  • 应用

    Functional Studies

    SDS-PAGE

  • 内毒素水平< 0.100 Eu/µg
  • 纯度> 95 % SDS-PAGE.
    Purity is greater than 98% by SDS-PAGE gel and HPLC analyses.
  • 形式Lyophilised
  • 补充说明Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶Reconstituted ab84082 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20oC. Avoid repeated freeze/thaw cycles.

常规信息

  • 别名
    • HGNC:5980
    • HsT19289
    • IL-16
    • IL16
    • IL16_HUMAN
    • Interleukin 16 precursor
    • Interleukin-16
    • LCF
    • Lymphocyte chemoattractant factor
    • Neuronal interleukin 16
    • NIL16
    • prIL 16
    • PrIL16
    • Prointerleukin 16
    see all
  • 功能Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.
    Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.
    Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.
  • 组织特异性Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T cell proliferation.
  • 序列相似性Contains 4 PDZ (DHR) domains.
  • 翻译后修饰Isoform 3 is synthesized as a chemo-attractant inactive precursor in hemopoietic tissues and is proteolytically cleaved by caspase-3 to yield IL-16.
  • 细胞定位Cytoplasm; Cytoplasm. Nucleus and Secreted.
  • Information by UniProt

Recombinant human IL16 protein 图像

  • The migration of human peripheral lymphocytes in response to human IL-16.
    Human peripheral blood lymphocytes were used in the chemotaxis assay at a final concentration of 5x106/ml.
    *p<0.001 compared to binding medium.

Recombinant human IL16 protein (ab84082)参考文献

ab84082 has not yet been referenced specifically in any publications.

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Thank you for contacting us.

The product can be reconstituted with sterile water.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclo...

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I am sorry for the delay in getting back to you. I now have some further information to share with you in regards to the migration assay.

I have attached a graph to this email illustrating the results which have been achieved with the protein...

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Until then, I wish you all the best with your research.

I am sorry for the delay in getting back to you. I now have further information to share with you in regards to the protocol used with the IL16 protein (Active) (ab84082). Please find the protocol attached to this email. The adjustments that need to be...

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Thank you for your enquiry.

As part of the QC for this particular antibody (ab84082), each batch is tested in a biological assay.Each batchmust meet the range of activity stated on the datasheet, therefore all the activities are the same and...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"