The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The ED50 as determined by the induction of IFN-γ production from murine splenocytes co-stimulated with IL-12 is <0.1 ng/ml, corresponding to a specific activity of > 1 x 107 units/mg.
The lyophilized human IL-12 is best-stored desiccated below 0oC. Reconstituted IL-12 should be stored at working aliquots at -20oC.
This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or –20oC for future use.
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: PBS, pH 7.2-7.4
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Centrifuge the vial prior to opening. Reconstitute in 1X PBS (pH 7.2-7.4) to a concentration of 0.2 mg/ml.