Recombinant人IL1 alpha protein (ab73567)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列SAPFSFLSNVKYNFMRIIKYEFILNDALNQSIIRANDQYLTAAALHNLDE AV KFDMGAYKSSKDDAKITVILRISKTQLYVTAQDEDQPVLLKEMPEI PKTITG SETNLLFFWETHGTKNYFTSVAHPNLFIATKQDYWVCLAGGPPSIT DFQILE NQA
    • 分子量18 kDa
    • 氨基酸113 to 271
    • 额外的序列信息Interleukin-1 alpha Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 159 amino acids and having a molecular mass of 18022 Dalton.

技术指标

Our Abpromise guarantee covers the use of ab73567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific Activity: 1 x 109 IU/mg.
  • 应用

    Functional Studies

    SDS-PAGE

    Western blot

  • 纯度> 95 % SDS-PAGE.
    ab73567 is purified by proprietary chromatographic techniques. Purity greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
  • 形式Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 5mM Magnesium chloride, 20mM Tris HCl, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶Reconstitute in sterile 18Mohm/cm H2O at not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

常规信息

  • 别名
    • BAF
    • FAF
    • Hematopoietin 1
    • Hematopoietin-1
    • IL 1 alpha
    • IL 1A
    • IL-1 alpha
    • Il-1a
    • IL1
    • IL1 ALPHA
    • IL1A
    • IL1A_HUMAN
    • IL1F1
    • Interleukin 1 alpha
    • Interleukin-1 alpha
    • Interleukin1 alpha
    • LAF
    • LEM
    • Preinterleukin 1 alpha
    • Pro interleukin 1 alpha
    see all
  • 功能Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • 序列相似性Belongs to the IL-1 family.
  • 结构域The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • 细胞定位Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt

Recombinant human IL1 alpha protein 图像

  • Anti-IL1 alpha antibody (ab9614) at 1 µg/ml + Recombinant human IL1 alpha protein (ab73567) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

Recombinant human IL1 alpha protein (ab73567)参考文献

ab73567 has not yet been referenced specifically in any publications.

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