Recombinant人IGFBP2 protein (ab63223)

概述

描述

  • 性质Recombinant
  • 来源Insect cells
  • 氨基酸序列
    • AccessionP18065
    • 种属Human
    • 序列EVLFRCPPCT PERLAACGPP PVAPPAAVAA VAGGARMPCA ELVREPGCGC CSVCARLEGE ACQVYTPRCG QGLRCYPHPG SELPLQALVM GEGTCEKRRD AEYGASPEQV ADNGDDHSEG GLVENHVDST MNMLGGGGSA GRKPLKSGMK ELAVFREKVT EQHRQMGKGG KHHLGLEEPK KLRPPPARTP CQQELDQVLE RISTMRLPDE RGPLEHLYSL HIPNCDKHGL YNLKQCKMSL NGQRGECWCV NPNTGKLIQG APTIRGDPEC HLFYNEQQEA RGVHTQRMQ
    • 分子量32 kDa
    • 氨基酸37 to 325
    • 额外的序列信息The sequence is the full length mature form minus the signal peptide.

技术指标

Our Abpromise guarantee covers the use of ab63223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性

    Biological Activity : Determined by its ability to inhibit IGF-I induced proliferation of FDC-P1 cells.

  • 应用

    Western blot

    Functional Studies

    SDS-PAGE

  • 内毒素水平< 1.000 Eu/µg
  • 纯度> 98 % SDS-PAGE.
    Greater than 98% by SDS-PAGE gel and HPLC analyses. Endotoxin level is < 0.1 ng/µg of protein (<1EU/µg).
  • 形式Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • BP 2
    • BP2
    • IBP 2
    • IBP-2
    • IBP2
    • IBP2_HUMAN
    • IGF binding protein 2
    • IGF BP53
    • IGF-binding protein 2
    • IGFBP 2
    • IGFBP-2
    • IGFBP2
    • IGFBP53
    • Insulin like growth factor binding protein 2
    • Insulin like growth factor binding protein 2 36kDa
    • Insulin like growth factor-binding protein 2 precursor
    • Insulin-like growth factor-binding protein 2
    see all
  • 功能Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • 序列相似性Contains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • 结构域The C-terminus is required for IGF-binding and growth inhibition.
  • 翻译后修饰O-glycosylated.
  • 细胞定位Secreted.
  • Information by UniProt

Recombinant human IGFBP2 protein 图像

  • Anti-IGFBP2 antibody (ab91404) at 1/500 dilution + Recombinant human IGFBP2 protein (ab63223) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

Recombinant human IGFBP2 protein (ab63223)参考文献

ab63223 has not yet been referenced specifically in any publications.

Product Wall

A serum-free media is used for culturing. However, please note that the virus stock contains serum and the final concentration of the serum is 0.1% FBS in the production.

This product is provided is free of any carrier proteins.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"