概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 序列
      AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE
    • 分子量
      8 kDa

相关产品

技术指标

Our Abpromise guarantee covers the use of ab9575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    The ED50 was determined by a cell proliferation assay using FDC-P1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.
  • 应用

    Functional Studies

    SDS-PAGE

  • 形式
    Lyophilised
  • 补充说明

    The ED50 was determined by a cell proliferation assay using FDC-P1 cells is < 2.0 ng/ml, corresponding to a specific activity of >5 x 105 units/mg.

    ab9575 was used as a positive control for ab9574 (Anti-IGF2 antibody).

     

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    n/a

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶
    Reconstitute with 100ul of water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.

常规信息

  • 别名
    • C11orf43
    • IGF 2
    • IGF II
    • IGF-II
    • IGF2
    • IGF2_HUMAN
    • IGFII
    • INSIGF
    • Insulin like Growth Factor 2
    • insulin like growth factor 2 (somatomedin A)
    • Insulin like growth factor II
    • Insulin like growth factor II precursor
    • Insulin like growth factor type 2
    • pp9974
    • Preptin
    • putative insulin like growth factor II associated protein
    • Somatomedin A
    • Somatomedin-A
    see all
  • 功能
    The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
    Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
  • 疾病相关
    Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations.
  • 序列相似性
    Belongs to the insulin family.
  • 翻译后修饰
    O-glycosylated with a core 1 or possibly core 8 glycan.
  • 细胞定位
    Secreted.
  • Information by UniProt

图片

  • Standard curve for IGF2 (Analyte: ab9575); dilution range 1pg/ml to 1µg/ml using Capture Antibody ab86485 at 1µg/ml and Detector Antibody ab18954 at 0.5µg/ml.

文献

This product has been referenced in:
  • Brouwer-Visser J  et al. Insulin-like growth factor 2 silencing restores taxol sensitivity in drug resistant ovarian cancer. PLoS One 9:e100165 (2014). Functional Studies . Read more (PubMed: 24932685) »
  • Suvasini R  et al. Insulin Growth Factor-2 Binding Protein 3 (IGF2BP3) Is a Glioblastoma-specific Marker That Activates Phosphatidylinositol 3-Kinase/Mitogen-activated Protein Kinase (PI3K/MAPK) Pathways by Modulating IGF-2. J Biol Chem 286:25882-90 (2011). Read more (PubMed: 21613208) »

See all 2 Publications for this product

客户评价及客户问答

We have several IGF2 antibodies but the immunogens for each are not all commercially available, and not all are raised against peptides. For the example I gave, ab9574, the immunogen is full-length recombinant IGF2, and this is available as ab9575: Read More

The blocking protein would be ab9575. Please see datasheet for specifics.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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