重组人IDI2蛋白(His tag) (ab219720)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 序列
      SDINLDWVDRRQLQRLEEMLIVVDENDKVIGADTKRNCHLNENIEKGLLH RAFSVVLFNTKNRILIQQRSDTKVTFPGYFTDSCSSHPLYNPAELEEKDA IGVRRAAQRRLQAELGIPGEQISPEDIVFMTIYHHKAKSDRIWGEHEICY LLLVRKNVTLNPDPSETKSILYLSQEELWELLEREARGEVKVTPWLRTIA ERFLYRWWPHLDDVTPFVELHKIHRV
    • 分子量
      28 kDa including tags
    • 氨基酸
      2 to 227
    • 标签
      His tag N-Terminus
    • 额外的序列信息
      (Accession # AAH17778).

技术指标

Our Abpromise guarantee covers the use of ab219720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 内毒素水平
    < 1.000 Eu/µg
  • 纯度
    >95% by SDS-PAGE .

  • 形式
    Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.87% Sodium chloride, 0.61% Tris buffered saline, 0.002% PMSF, 0.02% DTT

    Note: 5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%.

  • 复溶
    It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 1mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

常规信息

  • 别名
    • Diphosphate dimethylallyl diphosphate isomerase 2
    • IDI 2
    • IDI2
    • IDI2_HUMAN
    • iIsopentenyl diphosphate delta isomerase 2
    • IPP isomerase 2
    • IPPI 2
    • IPPI2
    • Isopentenyl diphosphate Delta isomerase 2
    • Isopentenyl pyrophosphate isomerase 2
    • Isopentenyl-diphosphate Delta-isomerase 2
    • OTTHUMP00000018952
    see all
  • 功能
    Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP).
  • 组织特异性
    Detected in skeletal muscle.
  • 通路
    Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from isopentenyl diphosphate: step 1/1.
  • 序列相似性
    Belongs to the IPP isomerase type 1 family.
    Contains 1 nudix hydrolase domain.
  • 细胞定位
    Peroxisome.
  • Information by UniProt

图片

  • SDS-PAGE analysis of reduced ab219720 stained overnight with Coomassie Blue.

文献

ab219720 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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