Recombinant人HUS1 protein (ab101944)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionO60921
    • 种属Human
    • 序列MGSSHHHHHHSSGLVPRGSHMKFRAKIVDGACLNHFTRISNMIAKLAKTC TLRISPDKLNFILCDKLANGGVSMWCELEQENFFNEFQMEGVSAENNEIY LELTSENLSRALKTAQNARALKIKLTNKHFPCLTVSVELLSMSSSSRIVT HDIPIKVIPRKLWKDLQEPVVPDPDVSIYLPVLKTMKSVVEKMKNISNHL VIEANLDGELNLKIETELVCVTTHFKDLGNPPLASESTHEDRNVEHMAEV HIDIRKLLQFLAGQQVNPTKALCNIVNNKMVHFDLLHEDVSLQYFIPALS
    • 分子量34 kDa including tags
    • 氨基酸1 to 280
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab101944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Mass Spectrometry

  • 质谱法
    MALDI-TOF
  • 纯度> 95 % SDS-PAGE.
    ab101944 is purified using conventional chromatography techniques.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 40% Glycerol, 20mM Tris HCl, 100mM Sodium chloride, pH 8.0

常规信息

  • 别名
    • Checkpoint protein HUS1
    • hHUS1
    • Hus1
    • HUS1 (S. pombe) checkpoint homolog
    • HUS1 checkpoint homolog (S. pombe)
    • HUS1 Checkpoint Protein
    • HUS1 protein
    • HUS1+ - like protein
    • HUS1_HUMAN
    • Hydroxyurea-sensitive 1, S. pombe, homolog of
    see all
  • 功能Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the HUS1 family.
  • 细胞定位Nucleus. Cytoplasm. In discrete nuclear foci upon DNA damage. According to PubMed:14500360, localized also in the cytoplasm. DNA damage induces its nuclear translocation. Shuttles between the nucleus and the cytoplasm.
  • Information by UniProt

Recombinant Human HUS1 protein 图像

  • 15% SDS-PAGE analysis of 3µg ab101944.

Recombinant Human HUS1 protein (ab101944)参考文献

ab101944 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us and I am sorry for the delay in getting back to you.

As mentioned in my previous email I have contacted the lab in regards to the HUS1 protein, ab101944 in order to try to understand why the results you have observe...

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Thank you for your reply and for sharing that extra information.

From the details provided by the customer I cannot explain why they are not able to see the protein by SDS-PAGE when staining with the Coomassie stain. If they would like, I can ...

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I am sorry for the delay in getting back to you. I now have further information to share with you from the lab in regards to the HUS1 protein (ab101944).

Following the concerns raised by you, the protein has been re-run on an SDS-PAGE gel to o...

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In orde...

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