重组人Hsp90 beta蛋白(ab80033)

概述

描述

  • 性质
    Recombinant
  • 来源
    Baculovirus infected BTI-TN-5B1-4 cells
  • 氨基酸序列
    • 种属
      Human

相关产品

技术指标

Our Abpromise guarantee covers the use of ab80033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性

    1. The reactions are in 33 mM Hepes pH7.2, 30 mM NaCl, 5 mM MgCl2, 1 mM DTT, 1.5 mM ATP in a 100 µl reaction at 37ºC. It is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm.

    2. The protein concentrations (where applicable) were 2 µM Hsp90 (Hsp90 beta), 2 µM Ahsa1 (His tagged human Ahsa1), 4 µM p23 (cleaved human p23), 4 µM HOP (His-tagged human HOP).

    3. Addition of a two-fold excess of p23 reduced Aha1-mediated Hsp90 stimulation by 30% (when using 2 µM Hsp90 and Aha1).

    4. Addition of a two -fold excess of HOP completely eliminated Aha1-mediated ATPase stimulation as well as intrinsic Hsp90 ATPase activity.

  • 应用

    Western blot

    ELISA

    Inhibition Assay

    Functional Studies

    SDS-PAGE

  • 纯度
    > 90 % SDS-PAGE.
    ab80033 is Multi-step chromatographically purified.
  • 形式
    Liquid
  • 补充说明
    ab80033 is endotoxin free. <0.1 EU/ml.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris, 175mM Sodium chloride, 1mM DTT, 0.1mM EDTA, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • 90 kda heat shock protein beta HSP90 beta
    • D6S182
    • FLJ26984
    • Heat shock 84 kDa
    • Heat shock 90kD protein 1, beta
    • Heat shock 90kDa protein 1 beta
    • Heat shock protein 90 alpha family class B member 1
    • Heat shock protein 90 kDa
    • Heat shock protein 90kDa alpha (cytosolic) class B member 1
    • Heat shock protein 90kDa alpha family class B member 1
    • Heat shock protein beta
    • Heat shock protein HSP 90 beta
    • Heat shock protein HSP 90-beta
    • HS90B_HUMAN
    • HSP 84
    • HSP 90
    • HSP 90 b
    • HSP 90b
    • HSP84
    • HSP90 BETA
    • hsp90ab1
    • HSP90B
    • HSPC2
    • HSPCB
    see all
  • 功能
    Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
  • 序列相似性
    Belongs to the heat shock protein 90 family.
  • 结构域
    The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins.
  • 翻译后修饰
    Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
    ISGylated.
    S-nitrosylated; negatively regulates the ATPase activity.
  • 细胞定位
    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

图片

文献

ab80033 has not yet been referenced specifically in any publications.

客户评价及客户问答


The ab80033 Recombinant human Hsp90 beta protein does not have any tags.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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