Recombinant人Hsp70 protein (ab78427)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 序列
      MKLSLVAAMLLLLSAARAEEEDKKEDVGTVVGIDLGTTYSCVGV FKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFD AKRLIGRT WNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTK MKETAEAY LGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGL
    • 氨基酸
      1 to 193
    • 额外的序列信息
      corresponds to Heat shock 70 kDa protein 5 (SwissProt P11021)

技术指标

Our Abpromise guarantee covers the use of ab78427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    ab78427 has ATPase activity at the time of manufacture of 3.3µM phosphate liberated/hr/µg protein in a 200µl reaction at 37°C (pH7.5) in the presence of 20ul of 1mM ATP using a Malachite Green assay.
  • 应用

    Functional Studies

    Other

    Western blot

    ELISA

    SDS-PAGE

  • 纯度
    > 90 % SDS-PAGE.
    ab78427 is affinity purified.
  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    Preservative: 200mM Imidazole
    Constituents: 10% Glycerol, 20mM Sodium phosphate, 150mM Sodium chloride, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • DnaK type molecular chaperone HSP70 1
    • Epididymis secretory protein Li 103
    • FLJ54303
    • FLJ54370
    • FLJ54392
    • FLJ54408
    • FLJ75127
    • Heat shock 70 kDa protein 1
    • Heat shock 70 kDa protein 1/2
    • Heat shock 70 kDa protein 1A/1B
    • Heat shock 70kDa protein 1A
    • Heat shock 70kDa protein 1B
    • Heat shock induced protein
    • HEL S 103
    • HSP70 1
    • HSP70 1B
    • HSP70 2
    • HSP70-1/HSP70-2
    • HSP70-1A
    • HSP70.1
    • HSP70.1/HSP70.2
    • HSP70I
    • HSP71_HUMAN
    • HSP72
    • HSPA1
    • HSPA1A
    • HSPA1B
    see all
  • 功能
    In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.
  • 组织特异性
    HSPA1B is testis-specific.
  • 序列相似性
    Belongs to the heat shock protein 70 family.
  • 细胞定位
    Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt

图片

  • Lane 1. Hsp70 protein

    Lane 2. Molecular weight ladder

  • Anti-Hsp70 antibody (ab94368) at 1 µg/ml + Recombinant human Hsp70 protein (ab78427) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 90 seconds

文献

ab78427 has not yet been referenced specifically in any publications.

客户评价及客户问答

When we tested this protein we loaded 10ul of protein solution with stock 0.01ug/ul concentration. (0.1ug/10ul).

We are selling this protein at concentration 0.47ug/ul. We recommend customer using 2ul of 0.47ug/ul and then adding 8ul of diluen...

Read More

Thank you for contacting us.

The protein showed ˜75 kDa band.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. E...

Read More

This protein is produced in E. coli BL21 cells and purified on a nickel column, eluted with imidazole using a his tag. Thus you should be able to use an anti-his tag antibody to detect this protein.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册