Recombinant人Hsp47 protein (ab92439)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human

技术指标

Our Abpromise guarantee covers the use of ab92439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    SDS-PAGE

    ELISA

  • 纯度> 90 % SDS-PAGE.
    Purified under denaturing conditions from the inclusion body fraction by multi-step chromatography. Endotoxin: <50 EU/mg as determined by LAL gel clot assay
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 30mM Tris, 100mM Sodium chloride, 1mM DTT, 0.4M Arginine, pH 8.0

常规信息

  • 别名
    • 47 kDa heat shock protein
    • 47 kDa heat shock protein precursor
    • Arsenic transactivated protein 3
    • Arsenic-transactivated protein 3
    • AsTP 3
    • AsTP3
    • CBP 1
    • CBP 2
    • CBP1
    • CBP2
    • Cell proliferation-inducing gene 14 protein
    • Collagen binding protein
    • Collagen binding protein 1
    • Collagen binding protein 2
    • Collagen-binding protein
    • Collagen-binding protein 2
    • Colligen
    • Colligin
    • Colligin 1
    • Colligin 2
    • colligin-1
    • colligin-2
    • gp46
    • Heat shock protein 47
    • Heat-shock protein 47
    • HGNC 1547
    • Hsp 47
    • J6
    • OI10
    • PIG 14
    • PIG14
    • PPROM
    • Proliferation inducing gene 14
    • Proliferation inducing gene 14 protein
    • RA A47
    • RA-A47
    • Rheumatoid arthritis antigen A 47
    • rheumatoid arthritis antigen A-47
    • Rheumatoid arthritis related antigen RA A47
    • Rheumatoid arthritis-related antigen RA-A47
    • serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
    • serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2)
    • Serine or cysteine proteinase inhibitor clade H member 1
    • Serine or cysteine proteinase inhibitor clade H member 2
    • SERPH_HUMAN
    • Serpin H1
    • Serpin peptidase inhibitor clade H member 1
    • serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
    • Serpin peptidase inhibitor, clade H, member 1
    • SERPINH1
    • SERPINH2
    see all
  • 功能Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
  • 疾病相关Note=Defects in SERPINH1 may cause severe autosomal recessive osteogenesis imperfecta (OI). Osteogenesis imperfecta defines a group of connective tissue disorders characterized by bone fragility and low bone mass.
  • 序列相似性Belongs to the serpin family.
  • 细胞定位Endoplasmic reticulum lumen.
  • Information by UniProt

Recombinant Human Hsp47 protein 图像

  • Anti-Hsp47 antibody (ab77609) at 1 µg/ml + Recombinant Human Hsp47 protein (ab92439) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds
  • SDS-PAGE Analysis. Lane 1: MW marker, Lane 2: 1.0 µg of purified Hsp47 (ab92439).

Recombinant Human Hsp47 protein (ab92439)参考文献

ab92439 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application ELISA

Review text: We used this protein to assess its binding to procollagen-coated plates, and test any Hsp47 binding inhibitor candidates that we have in our lab.
Sample: Human Purified protein (procollagen purified from human skin fibroblast cu)
Specification: procollagen purified from human skin fibroblast cu
Blocking step: BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Type: Indirect

Other product details
Dilution: 1/1000
Incubation time: 1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 2% BSA and 0.05% Tween-20 in PBS

Primary antibody (in addition to 'Hsp47 protein')
Dilution: 1/2000

Secondary antibody
Name: Non-Abcam antibody was used: goat anti-rabbit AP-conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Dilution: 1/10000
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提交于 Nov 05 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"