Recombinant人HSF1 (phospho ) protein (ab115508)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionQ00613
    • 种属Human
    • 分子量70 kDa including tags
    • 氨基酸1 to 529
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab115508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Western blot

  • 纯度> 90 % SDS-PAGE.
    Purity =90% (~60% full-length with trace upstream kinase).
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Constituents: 5% Glycerol, 99% Tris buffered saline

常规信息

  • 别名
    • Heat shock factor 1
    • Heat shock factor protein 1
    • Heat shock transcription factor 1
    • HSF 1
    • hsf1
    • HSF1_HUMAN
    • HSTF 1
    • HSTF1
    see all
  • 功能DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
  • 序列相似性Belongs to the HSF family.
  • 结构域the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • 翻译后修饰Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
    Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.
  • 细胞定位Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
  • Information by UniProt

Recombinant Human HSF1 (phospho ) protein 图像

  • SDS-PAGE analysis:
    Lane 1: MWM,
    Lane 2: 2µg HSF1,
    Lane 3: 2µg ab115508 (HSF1 - phospho)


  • Predicted band size : 60 kDa


  • Predicted band size : 60 kDa

Recombinant Human HSF1 (phospho ) protein (ab115508)参考文献

ab115508 has not yet been referenced specifically in any publications.

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I have heard back from the lab, and they have confirmed that ab117515 is phosphorylated in vitro to produce ab115508.
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