Recombinant人HSD3a protein (ab109831)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP17516
    • 种属Human
    • 序列MGSSHHHHHHSSGLVPRGSHMDPKYQRVELNDGHFMPVLGFGTYAPPEVP RNRAVEVTKLAIEAGFRHIDSAYLYNNEEQVGLAIRSKIADGSVKREDIF YTSKLWCTFFQPQMVQPALESSLKKLQLDYVDLYLLHFPMALKPGETPLP KDENGKVIFDTVDLSATWEVMEKCKDAGLAKSIGVSNFNCRQLEMILNKP GLKYKPVCNQVECHPYLNQSKLLDFCKSKDIVLVAHSALGTQRHKLWVDP NSPVLLEDPVLCALAKKHKRTPALIALRYQLQRGVVVLAKSYNEQRIREN IQVFEFQLTSEDMKVLDGLNRNYRYVVMDFLMDHPDYPFSDEY
    • 分子量39 kDa including tags
    • 氨基酸1 to 343
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab109831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性

    Specific activity is > 700 pmol/min/μg, and is defined as the amount of enzyme that catalyzes the reduction of 1.0 pmole 3-chlorobenzaldehyde in the presence of NADP per minute at pH 8.8 at 25°C.

  • 应用

    Functional Studies

    SDS-PAGE

    Mass Spectrometry

  • 质谱法
    MALDI-TOF
  • 纯度> 90 % SDS-PAGE.
    ab109831 was purified using conventional chromatography.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20% Glycerol, 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% DTT, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • 3 alpha HSD1
    • 3-alpha-HSD1
    • 3-alpha-hydroxysteroid dehydrogenase type I
    • AK1C4_HUMAN
    • AKR1C4
    • Aldo keto reductase family 1 member C4
    • Aldo-keto reductase family 1 member C4
    • CDR
    • Chlordecone reductase
    • DD-4
    • DD4
    • Dihydrodiol dehydrogenase 4
    • HAKRA
    see all
  • 功能Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol). Also has some 20-alpha-hydroxysteroid dehydrogenase activity. The biotransformation of the pesticide chlordecone (kepone) to its corresponding alcohol leads to increased biliary excretion of the pesticide and concomitant reduction of its neurotoxicity since bile is the major excretory route.
  • 组织特异性Liver specific.
  • 序列相似性Belongs to the aldo/keto reductase family.
  • 翻译后修饰The N-terminus is blocked.
  • 细胞定位Cytoplasm.
  • Information by UniProt

Recombinant human HSD3a protein 图像

  • 15% SDS-PAGE image, using ab109831 at 3ug

Recombinant human HSD3a protein (ab109831)参考文献

ab109831 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"