Recombinant人Hsc70 protein (ab78431)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 分子量73 kDa including tags

技术指标

Our Abpromise guarantee covers the use of ab78431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性ab78431 has ATPase activity at the time of manufacture of 3.2µM phosphate liberated/hr/µg protein in a 200µl reaction at 37°C (pH7.5) in the presence of 20ul of 1mM ATP using a Malachite Green assay.
  • 应用

    Western blot

    ELISA

    Functional Studies

    competitive binding assays

    SDS-PAGE

  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    Preservative: 200mM Imidazole
    Constituents: 10% Glycerol, 20mM Sodium phosphate, 150mM Sodium chloride, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • 2410008N15Rik
    • Constitutive heat shock protein 70
    • Epididymis luminal protein 33
    • Epididymis secretory sperm binding protein Li 72p
    • Heat shock 70 kDa protein 8
    • Heat shock 70kD protein 10
    • Heat shock 70kD protein 8
    • Heat shock 70kDa protein 8
    • Heat shock cognate 71 kDa protein
    • Heat shock cognate protein 54
    • Heat shock cognate protein 71 kDa
    • Heat shock protein 8
    • Heat shock protein A8
    • Heat-shock70-KD protein 10, formerly
    • HEL 33
    • HEL S 72p
    • HSC54
    • HSC71
    • Hsc73
    • HSP71
    • HSP73
    • HSP7C_HUMAN
    • HSPA10
    • HSPA8
    • LAP1
    • Lipopolysaccharide associated protein 1
    • LPS associated protein
    • LPS associated protein 1
    • MGC102007
    • MGC106514
    • MGC114311
    • MGC118485
    • MGC131511
    • MGC29929
    • N-myristoyltransferase inhibitor protein 71
    • NIP71
    see all
  • 功能Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the heat shock protein 70 family.
  • 结构域The N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    ISGylated.
  • 细胞定位Cytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

Recombinant human Hsc70 protein 图像

  • Lane 1. Molecular weight ladder

    Lane 2. Hsc70 protein

Recombinant human Hsc70 protein (ab78431)参考文献

ab78431 has not yet been referenced specifically in any publications.

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