The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
生物活性ab78431 has ATPase activity at the time of manufacture of 3.2µM phosphate liberated/hr/µg protein in a 200µl reaction at 37°C (pH7.5) in the presence of 20ul of 1mM ATP using a Malachite Green assay.
competitive binding assays
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Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Constitutive heat shock protein 70
Epididymis luminal protein 33
Epididymis secretory sperm binding protein Li 72p
Heat shock 70 kDa protein 8
Heat shock 70kD protein 10
Heat shock 70kD protein 8
Heat shock 70kDa protein 8
Heat shock cognate 71 kDa protein
Heat shock cognate protein 54
Heat shock cognate protein 71 kDa
Heat shock protein 8
Heat shock protein A8
Heat-shock70-KD protein 10, formerly
HEL S 72p
Lipopolysaccharide associated protein 1
LPS associated protein
LPS associated protein 1
N-myristoyltransferase inhibitor protein 71
功能Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
序列相似性Belongs to the heat shock protein 70 family.
结构域The N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.
翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR. ISGylated.
细胞定位Cytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.