Recombinant人Histone H2B protein (ab92409)



  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human


Our Abpromise guarantee covers the use of ab92409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    Functional Studies

  • 纯度>= 95 % SDS-PAGE.

  • 形式Liquid
  • Concentration information loading...


  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: Dulbecco's PBS


  • 别名
    • H2B GL105
    • H2B histone family member O
    • H2B histone family member S
    • H2B.1
    • H2B.1 B
    • H2B.b
    • H2B.c
    • H2B.d
    • H2B.e
    • H2B.f
    • H2B.j
    • H2B.q
    • H2B/b
    • H2B/c
    • H2B/d
    • H2B/e
    • H2B/f
    • H2B/j
    • H2B/o
    • H2B/q
    • H2BFB
    • H2BFC
    • H2BFD
    • H2BFE
    • H2BFF
    • H2BFJ
    • H2BFO
    • H2BFQ
    • H2BFS
    • HIRIP2
    • HIST1H2BB
    • HIST1H2BD
    • HIST1H2BH
    • HIST1H2BL
    • HIST1H2BM
    • HIST1H2BN
    • HIST2H2BE
    • Histone H2B
    • Histone H2B type 1 B
    • Histone H2B type 1 D
    • Histone H2B type 1 H
    • Histone H2B type 1 L
    • Histone H2B type 1 M
    • Histone H2B type 1 N
    • Histone H2B type 2 E
    • histone protein
    see all
  • 相关性Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
  • 细胞定位Nuclear

Recombinant Human Histone H2B protein (ab92409)参考文献

ab92409 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry and for your patience.

As discussed in my previous email, I can confirm that this particular product is sourced externally. I have been in contact with them in order to obtain this information for you, and regretta...

Read More