The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
生物活性Specific activity is 3-4 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. In the coupled mode, one unit will produce 1.0 umole of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 30C.
% SDS-PAGE. ab85920 is prified using conventional chromatography techniques.
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 10% Glycerol, 20mM Tris HCl, pH 8.0
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Hexokinase 2 muscle
Hexokinase type II
Muscle form hexokinase
组织特异性Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.
通路Carbohydrate metabolism; hexose metabolism.
序列相似性Belongs to the hexokinase family. Contains 2 hexokinase domains.
结构域The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
细胞定位Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.