Recombinant人HDAC5 protein (ab80351)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus
  • 氨基酸序列
    • AccessionQ9UQL6
    • 种属Human
    • 分子量51 kDa
    • 氨基酸656 to 1122
    • 标签His tag C-Terminus

技术指标

Our Abpromise guarantee covers the use of ab80351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    Specific Activity: ≥1000 pmol/min/µg.
    Unit Definition: One U =1 pmol of acetyl group removed/min/µg of enzyme.
    Assay Conditions:
    25 mM Tris HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 30 µM HDAC class 2a substrate, and HDAC5. Incubation condition: 30 min at 30°C, followed by HDAC developer for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.

     

  • 应用

    SDS-PAGE

  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 50mM Tris HCl, 138mM Sodium chloride, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Antigen NY CO 9
    • Antigen NY-CO-9
    • HD5
    • HDAC 5
    • HDAC5
    • HDAC5_HUMAN
    • Histone deacetylase 5
    • NY CO 9
    see all
  • 功能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the histone deacetylase family. HD type 2 subfamily.
  • 结构域The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • 翻译后修饰Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
    Ubiquitinated. Polyubiquitination however does not lead to its degradation.
  • 细胞定位Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.
  • Information by UniProt

Recombinant human HDAC5 protein (ab80351)参考文献

ab80351 has not yet been referenced specifically in any publications.

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