Recombinant人HDAC2 protein (ab101662)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus
  • 氨基酸序列
    • AccessionQ92769
    • 种属Human
    • 分子量92 kDa including tags
    • 氨基酸1 to 488

技术指标

Our Abpromise guarantee covers the use of ab101662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    Functional Studies

  • 纯度> 70 % SDS-PAGE.
    The purity of ab101662 was determined to be >70% by densitometry.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

常规信息

  • 别名
    • D10Wsu179e
    • HD 2
    • HD2
    • HDAC 2
    • Hdac2
    • HDAC2_HUMAN
    • Histone deacetylase 2
    • Histone deacetylase 2 (HD2)
    • OTTHUMP00000017046
    • OTTHUMP00000227077
    • OTTHUMP00000227078
    • RPD3
    • transcriptional regulator homolog RPD3
    • YAF1
    • YY1 associated factor 1
    • YY1 transcription factor binding protein
    • Yy1bp
    see all
  • 功能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
    Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity.
  • 组织特异性Widely expressed; lower levels in brain and lung.
  • 序列相似性Belongs to the histone deacetylase family. HD type 1 subfamily.
  • 翻译后修饰S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.
  • 细胞定位Nucleus.
  • Information by UniProt

Recombinant Human HDAC2 protein 图像

  • The purity of ab101662 was determined to be >70% as determined by densitometry.
    Approximate molecular weight is ~92 kDa (with tag).
  • The specific activity of ab101662 was determined to be 4180 RLU/min/ng.

Recombinant Human HDAC2 protein (ab101662)参考文献

ab101662 has not yet been referenced specifically in any publications.

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