Recombinant人HDAC1 protein (ab101661)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus
  • 氨基酸序列
    • AccessionQ13547
    • 种属Human
    • 分子量88 kDa including tags
    • 氨基酸1 to 482

技术指标

Our Abpromise guarantee covers the use of ab101661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性The Specific activity of ab101661 was determined to be 8900 RLU/min/ng.
  • 应用

    Functional Studies

    Western blot

  • 纯度> 70 % SDS-PAGE.
    >70% by densitometry
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • DKFZp686H12203
    • GON 10
    • HD1
    • HDAC 1
    • HDAC1
    • HDAC1_HUMAN
    • Histone deacetylase 1
    • Reduced potassium dependency yeast homolog like 1
    • RPD3
    • RPD3L1
    see all
  • 功能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
  • 组织特异性Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
  • 序列相似性Belongs to the histone deacetylase family. HD type 1 subfamily.
  • 翻译后修饰Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
    Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome.
  • 细胞定位Nucleus.
  • Information by UniProt

Recombinant human HDAC1 protein 图像

  • SDS-PAGE showing ab101661 at approximately 88kDa.
  • The Specific activity of ab101661 was determined to be 8900 RLU/min/ng.

Recombinant human HDAC1 protein (ab101661)参考文献

ab101661 has not yet been referenced specifically in any publications.

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